The N-glycan profile of the peritrophic membrane in the Colorado potato beetle larva (Leptinotarsa decemlineata)

Dongdong Liu; Kristof De Schutter; Nicolas Smargiasso; Edwin De Pauw; Els J M Van Damme; Guy Smagghe

doi:10.1016/j.jinsphys.2019.03.009

The N-glycan profile of the peritrophic membrane in the Colorado potato beetle larva (Leptinotarsa decemlineata)

J Insect Physiol. 2019 May-Jun:115:27-32. doi: 10.1016/j.jinsphys.2019.03.009. Epub 2019 Mar 29.

Authors

Dongdong Liu¹, Kristof De Schutter², Nicolas Smargiasso³, Edwin De Pauw⁴, Els J M Van Damme³, Guy Smagghe⁵

Affiliations

¹ Laboratory of Agrozoology, Department Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium; Laboratory of Biochemistry and Glycobiology, Department of Biotechnology, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.
² Laboratory of Agrozoology, Department Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.
³ Laboratory of Biochemistry and Glycobiology, Department of Biotechnology, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.
⁴ Mass Spectrometry Laboratory, GIGA-Research, University of Liege, Liege, Belgium.
⁵ Laboratory of Agrozoology, Department Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium. Electronic address: guy.Smagghe@ugent.be.

PMID: 30935980
DOI: 10.1016/j.jinsphys.2019.03.009

Abstract

The insect peritrophic membrane (PM) is a non-cellular structure composed of secreted proteins imbedded in a proteoglycan matrix together with chitin. It separates the midgut epithelium from the intestinal contents, and functions in the digestion of food. Furthermore it acts as a protective barrier against abrasive particles and microbial infections. Here we studied for the first time the N-glycome of the PM. We identified the N-glycan structures present in the PM of the Colorado potato beetle (CPB) at the fourth larval stage using MALDI-TOF mass spectrometry. In parallel, we correlated the N-glycan data to the presence of the N-glycosylation related genes (NGRGs) in the transcriptome of epithelial midgut cells. The presumed activities of the identified genes support the N-glycan profile resolved for the proteins in the PM. To our knowledge these data are the first report on the N-glycome of the PM of a pest insect. These results will contribute to the study of the importance of N-glycosylation in the function and structure of the PM. In addition, the data can help to find novel targets and design better biopesticides for pest control.

Keywords: Leptinotarsa decemlineata; Midgut; N-glycome; N-glycosylation; Peritrophic membrane; Transcriptome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Coleoptera / metabolism*
Gastrointestinal Tract / metabolism
Glycosylation
Larva / metabolism
Polysaccharides / metabolism*
Transcriptome

Substances

Polysaccharides