Design of stable magnetic hybrid nanoparticles of Si-entrapped HRP

PLoS One. 2019 Apr 1;14(4):e0214004. doi: 10.1371/journal.pone.0214004. eCollection 2019.

Abstract

Hybrid and composite nanoparticles represent an attractive material for enzyme integration due to possible synergic advantages of the structural builders in the properties of the nanobiocatalyst. In this study, we report the synthesis of a new stable hybrid nanobiocatalyst formed by biomimetic silica (Si) nanoparticles entrapping both Horseradish Peroxidase (HRP) (EC 1.11.1.7) and magnetic nanoparticles (MNPs). We have demonstrated that tailoring of the synthetic reagents and post immobilization treatments greatly impacted physical and biocatalytic properties such as an unprecedented ~280 times increase in the half-life time in thermal stability experiments. The optimized nanohybrid biocatalyst that showed superparamagnetic behaviour, was effective in the batch conversion of indole-3-acetic acid, a prodrug used in Direct Enzyme Prodrug Therapy (DEPT). Our system, that was not cytotoxic per se, showed enhanced cytotoxic activity in the presence of the prodrug towards HCT-116, a colorectal cancer cell line. The strategy developed proved to be effective in obtaining a stabilized nanobiocatalyst combining three different organic/inorganic materials with potential in DEPT and other biotechnological applications.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / administration & dosage
  • Antineoplastic Agents / metabolism
  • Biocatalysis
  • Drug Delivery Systems / methods*
  • Drug Evaluation, Preclinical
  • Enzymes, Immobilized / chemistry*
  • Enzymes, Immobilized / metabolism
  • HCT116 Cells
  • Half-Life
  • Horseradish Peroxidase / chemistry*
  • Horseradish Peroxidase / metabolism
  • Humans
  • Indoleacetic Acids / administration & dosage
  • Indoleacetic Acids / metabolism
  • Magnetite Nanoparticles / chemistry
  • Nanocomposites / chemistry*
  • Prodrugs / administration & dosage
  • Prodrugs / metabolism
  • Silicon Dioxide / chemistry

Substances

  • Antineoplastic Agents
  • Enzymes, Immobilized
  • Indoleacetic Acids
  • Magnetite Nanoparticles
  • Prodrugs
  • indoleacetic acid
  • Silicon Dioxide
  • Horseradish Peroxidase

Grants and funding

SC is funded by ANII (POS_NAC_2015_1_109910).