Two previously undescribed guaiane-type sesquiterpenes (1 and 2), a pair of new salvialane-type sesquiterpenes (3a and 3b), together with 11 known compounds were isolated and purified from the rhizomes of Curcuma kwangsiensis. Their structures were elucidated by the extensive spectroscopic data (1D- and 2D-NMR) analysis. All the isolated compounds were assessed for their anti-neuroinflammatory activity by inhibiting the nitric oxide (NO) production in lipopolysaccharide (LPS)-activated murine BV-2 microglial cells in vitro assay, and the isolates 3 and 11 showed anti-neuroinflammatory activity with IC50 values of 1.85 and 20.05 μm, respectively.
Keywords: BV-2 microglial cells; Curcuma kwangsiensis; biological activity; guaiane-type sesquiterpene; nitric oxide; phytochemistry; salvialane-type sesquiterpene.
© 2019 Wiley-VHCA AG, Zurich, Switzerland.