Biosynthesis of 2,5-furan dicarboxylic acid by Aspergillus flavus APLS-1: Process optimization and intermediate product analysis

Rajendran Omana Rajesh; Tharangattumana Krishnan Godan; Amit Kumar Rai; Dinabandhu Sahoo; Ashok Pandey; Parameswaran Binod

doi:10.1016/j.biortech.2019.03.105

Biosynthesis of 2,5-furan dicarboxylic acid by Aspergillus flavus APLS-1: Process optimization and intermediate product analysis

Bioresour Technol. 2019 Jul:284:155-160. doi: 10.1016/j.biortech.2019.03.105. Epub 2019 Mar 21.

Authors

Rajendran Omana Rajesh¹, Tharangattumana Krishnan Godan¹, Amit Kumar Rai², Dinabandhu Sahoo², Ashok Pandey³, Parameswaran Binod⁴

Affiliations

¹ Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Thiruvananthapuram 695019, Kerala, India; Academy of Scientific and Innovative Research (AcSIR), CSIR-National Institute for Interdisciplinary Science and Technology, Thiruvananthapuram 695019, Kerala, India.
² Institute of Bioresources and Sustainable Development, Sikkim Centre, Tadong - 737102, Gangtok, Sikkim, India.
³ Centre for Innovation and Translational Research, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), 31 MG Marg, Lucknow 226 001, India.
⁴ Microbial Processes and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology, Thiruvananthapuram 695019, Kerala, India. Electronic address: binodkannur@niist.res.in.

PMID: 30928827
DOI: 10.1016/j.biortech.2019.03.105

Abstract

The aim of the present study was to develop an eco-friendly biological process for the production of 2,5-furan dicarboxylic acid (FDCA) from 5-hydroxy methylfurfuraldehyde (HMF) using microorganisms. Microorganisms were isolated from the soil samples and evaluated for its biotransformation efficiency. Among the isolates, Aspergillus flavus APLS-1 was found to be potent for efficient conversion of HMF to FDCA. The bioconversion parameters were optimized by Box-Behnken design. The optimization resulted in 67% conversion efficiency where 1 g/L HMF (8 mM) was transformed to 0.83 g/L (6.6 mM) FDCA in 14 days at pH6.5 with biomass size of 5.7 g/L and biomass age 60 h. This is the first report on Aspergillus sp., capable of detoxifying HMF and produces FDCA.

Keywords: 2,5-furan dicarboxylic acid; Aspergillus flavus; Biotransformation; Green bioprocess; Hydroxymethyl furfuraldehyde.

MeSH terms

Aspergillus flavus / metabolism*
Biomass
Biotransformation
Dicarboxylic Acids / metabolism*
Furans / metabolism*

Substances

Dicarboxylic Acids
Furans