Variable chemokine expression in porcine trophoblasts and endometrium during the peri-implantation period

Abstract

Successful embryo implantation and its further development depends on appropriate endometrial remodelling. Porcine early pregnancy is associated with intensive endometrial angiogenesis and establishment of an immunotolerant environment for the embryo. An increasing number of factors are believed to participate in endometrial remodelling. The aim of this study was to elucidate the involvement of selected chemokines at the porcine maternal-foetal interface during the peri-implantation period. Real-time PCR analysis revealed several upregulated chemokines during the time of implantation, and Western blot/ELISA analyses and immunohistochemical staining confirmed their presence at the protein level. The gene expression of several chemokines and receptors was also confirmed in early porcine trophoblasts. The results indicated that IFNG, a porcine trophoblast signal, positively influenced the expression of some chemokines in endometrial cells. In conclusion, we suggest that some of the examined chemokines may be involved in endometrial communication with the trophoblast (CCL2, CCL5, CCL11, CXCL12), whereas others are implicated in the recruitment of immune cells and establishment of an immunotolerant environment for the embryo (CXCL9, CXCL10).