Lack of P2X7 Receptors Protects against Renal Fibrosis after Pyelonephritis with α-Hemolysin-Producing Escherichia coli

Abstract

Severe urinary tract infections are commonly caused by sub-strains of Escherichia coli secreting the pore-forming virulence factor α-hemolysin (HlyA). Repeated or severe cases of pyelonephritis can cause renal scarring that subsequently can lead to progressive failure. We have previously demonstrated that HlyA releases cellular ATP directly through its membrane pore and that acute HlyA-induced cell damage is completely prevented by blocking ATP signaling. Local ATP signaling and P2X₇ receptor activation play a key role in the development of tissue fibrosis. This study investigated the effect of P2X₇ receptors on infection-induced renal scarring in a murine model of pyelonephritis. Pyelonephritis was induced by injecting 100 million HlyA-producing, uropathogenic E. coli into the urinary bladder of BALB/cJ mice. A similar degree of pyelonephritis and mortality was confirmed at day 5 after infection in P2X₇^+/+ and P2X₇^-/- mice. Fibrosis was first observed 2 weeks after infection, and the data clearly demonstrated that P2X₇^-/- mice and mice exposed to the P2X₇ antagonist, brillian blue G, show markedly less renal fibrosis 14 days after infection compared with controls (P < 0.001). Immunohistochemistry revealed comparable early neutrophil infiltration in the renal cortex from P2X₇^+/+ and P2X₇^-/- mice. Interestingly, lack of P2X₇ receptors resulted in diminished macrophage infiltration and reduced neutrophil clearance in the cortex of P2X₇^-/- mice. Hence, this study suggests the P2X₇ receptor to be an appealing antifibrotic target after renal infections.