DGCR8 expression is altered in children with congenital heart defects

Abstract

Aim: To explore the correlation of DGCR8 expression in children with congenital heart defects (CHD) and its clinical significance.

Methods: Full blood samples were collected from children with congenital heart disease(n = 40) and healthy children(n = 40), respectively.Real-time PCR was used to detect the expression of DGCR8 in the blood of healthy children and CHD. Myocardial tissues were collected from children with ventricular septal defect (VSD)(n = 25), and tetralogy of Fallot (TOF)(n = 16),. Real-time PCR and Western blotting were used to detect the expression of DGCR8 in myocardial tissues. Analyze the correlation between DGCR8 expression and congenital heart disease.

Results: The expression levels of DGCR8 was significantly lower in CHD than healthy children (P = 0.037), and lower in TOF tissues compared with VSD tissues (P = 0.046). There was no significant correlation between the expression of DGCR8 and the size of VSD(r = -0.022, P = 0.917).

Conclusions: The low expression of DGCR8 was significantly correlated with the occurrence of CHD, which may affect the development of heart and the formation of blood vessels. The lower expression of DGCR8 was correlated with severe CHD. However, DGCR8 expression did not associate with the size of VSD.

Keywords: Congenital heart disease; DGCR8; Tetralogy of fallot; Ventricular septal defect.