In Vitro Toxicity Study of a Porous Iron(III) Metal‒Organic Framework

Molecules. 2019 Mar 28;24(7):1211. doi: 10.3390/molecules24071211.

Abstract

A MIL series metal‒organic framework (MOF), MIL-100(Fe), was successfully synthesized at the nanoscale and fully characterized by TEM, TGA, XRD, FTIR, DLS, and BET. A toxicological assessment was performed using two different cell lines: human normal liver cells (HL-7702) and hepatocellular carcinoma (HepG2). In vitro cytotoxicity of MIL-100(Fe) was evaluated by the MTT assay, LDH releasing rate assay, DAPI staining, and annexin V/PI double staining assay. The safe dose of MIL-100(Fe) was 80 μg/mL. It exhibited good biocompatibility, low cytotoxicity, and high cell survival rate (HL-7702 cells' viability >85.97%, HepG2 cells' viability >91.20%). Therefore, MIL-100(Fe) has a potential application as a drug carrier.

Keywords: HL-7702 cells; HepG2 cells; MIL-100(Fe); in vitro toxicity.

MeSH terms

  • Apoptosis / drug effects
  • Cell Line
  • Cell Membrane / drug effects
  • Cell Membrane / metabolism
  • Cell Shape / drug effects
  • Cell Survival / drug effects
  • Humans
  • Iron / chemistry
  • Iron / toxicity*
  • Metal-Organic Frameworks / chemistry
  • Metal-Organic Frameworks / toxicity*
  • Metal-Organic Frameworks / ultrastructure
  • Models, Molecular
  • Particle Size
  • Porosity
  • Toxicity Tests*

Substances

  • Metal-Organic Frameworks
  • Iron