Modified mussel shell powder for microalgae immobilization to remove N and P from eutrophic wastewater

Lili Ji; Wendong Song; Danyi Wei; Dongjiao Jiang; Lu Cai; Yaning Wang; Jian Guo; Hailong Zhang

doi:10.1016/j.biortech.2019.03.112

Modified mussel shell powder for microalgae immobilization to remove N and P from eutrophic wastewater

Bioresour Technol. 2019 Jul:284:36-42. doi: 10.1016/j.biortech.2019.03.112. Epub 2019 Mar 22.

Authors

Lili Ji¹, Wendong Song², Danyi Wei³, Dongjiao Jiang⁴, Lu Cai⁵, Yaning Wang¹, Jian Guo⁶, Hailong Zhang⁷

Affiliations

¹ Institute of Innovation & Application, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China.
² College of Petrochemical and Energy Engineering College, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China.
³ College of Naval Architecture and Mechanical-Electrical Engineering, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China.
⁴ College of Marine Science and Technology, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China.
⁵ College of Environmental and Science Technology, Donghua University, Shanghai 201620, China.
⁶ College of Food and Medical, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China.
⁷ Institute of Innovation & Application, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China. Electronic address: h_l_zhang@yahoo.com.

PMID: 30925421
DOI: 10.1016/j.biortech.2019.03.112

Abstract

In this work, calcined mussel shell powder (CMSP) was activated by K₂CO₃ (K-CMSP), and this porous K-CMSP surface was modified by L-arginine (L-ARG) to render porous biomass a positively charged surface, which was innovatively utilized as a carrier to immobilize microalgae by adsorption via electrostatic interactions. The pore and the surface structures of CMSP and K-CMSP were characterized by XRD, FTIR, BET and SEM. The surface morphology of immobilized microalgae was visualized via using inverted optical microscope and SEM. It was found that microalgae could survive for 60 days, and the loss rate of chlorophyll-a preserved at -24 °C was the lowest, 44.73%. The microalgae could revive to normal growth level within 10 days and the cell content of microalgae was the highest at 25 °C, 2.8022 × 10⁶ cell/mL. At 25 °C, the highest removal rate of N and P was obtained about 95.0% and 88.63%, respectively.

Keywords: Calcined mussel shell powder; Immobilized; Microalgae; Modified; Water quality control agent.

MeSH terms

Animals
Biomass
Bivalvia*
Microalgae* / growth & development
Nitrogen / chemistry*
Phosphates / chemistry*
Wastewater / chemistry*

Substances

Phosphates
Waste Water
Nitrogen