Aim: Aim of this study was to evaluate the local effects of ozone gas on bacteria present within deep carious lesions after selective caries excavation.
Materials and methods: The present study included 75 patients who had at least two Class I caries with deep lesion D2 and/or D3. The cavities were divided into 2 groups: the ozone group received treatment with Healozone X4, while the control group did not receive any treatment. Dentin samples were taken from both groups using two sterile multi-blade drills at 800 rpm without irrigation. The drills were then stored in separate tubes, each containing 5ml of RTF (Reduced Transport Fluid) and placed in culture for the next two hours. A total microbial count of the CFU of Streptococcus Mutans and Lactobacillus was performed.
Conclusions: From the analysis of data on the dentin samples treated with ozone, there is a decrease in the bacterial load present in the infected carious dentin.