In situ hybridization assay for the diagnosis of chagas myocarditis and orchitis in a rhesus macaque (Macaca mulatta): A case report

Megan DeLorenzo; Elvira Carias; Allison Mustonen; Olga Gonzalez; Edward J Dick Jr; Shyamesh Kumar

doi:10.1111/jmp.12406

In situ hybridization assay for the diagnosis of chagas myocarditis and orchitis in a rhesus macaque (Macaca mulatta): A case report

J Med Primatol. 2019 Jun;48(3):182-185. doi: 10.1111/jmp.12406. Epub 2019 Mar 25.

Authors

Megan DeLorenzo^{1

2}, Elvira Carias^{1

3}, Allison Mustonen¹, Olga Gonzalez¹, Edward J Dick Jr¹, Shyamesh Kumar¹

Affiliations

¹ Southwest National Primate Research Center, Texas Biomedical Research Institute, San Antonio, Texas.
² College of Agriculture Forestry and Life Sciences, Clemson University, Clemson, South Carolina.
³ Microbiology and Immunology Program, University of Texas at San Antonio, San Antonio, Texas.

Abstract

We present the first documented case of Trypanosoma cruzi-induced orchitis in a rhesus macaque. Additionally, we describe an in situ hybridization-based assay to confirm T. cruzi infection in formalin-fixed tissues.

Keywords: PCR; RNAscope; kinetoplast; trypanosome.

Publication types

Case Reports

MeSH terms

Animals
Chagas Cardiomyopathy / diagnosis
Chagas Cardiomyopathy / parasitology
Chagas Cardiomyopathy / veterinary*
In Situ Hybridization / methods
In Situ Hybridization / veterinary*
Macaca mulatta*
Male
Monkey Diseases / diagnosis*
Monkey Diseases / parasitology
Orchitis / parasitology
Orchitis / veterinary*
Trypanosoma cruzi / isolation & purification*

Abstract

Publication types

MeSH terms

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