Genome Editing in Mice

Lisbeth Ahm Hansen; Ernst-Martin Füchtbauer

doi:10.1007/978-1-4939-9170-9_15

Genome Editing in Mice

Methods Mol Biol. 2019:1961:249-254. doi: 10.1007/978-1-4939-9170-9_15.

Authors

Lisbeth Ahm Hansen¹, Ernst-Martin Füchtbauer²

Affiliations

¹ Department of Biomedicine, Aarhus University, Aarhus, Denmark.
² Department of Molecular Biology and Genetics, Aarhus, Denmark. emf@mbg.au.dk.

PMID: 30912050
DOI: 10.1007/978-1-4939-9170-9_15

Abstract

Programmable nucleases like CRISPR/Cas9 enable to edit the mouse genome directly in the zygote. Several methods have been successfully used for this. Here we describe injection into one of the pronuclei of the zygote and electroporation of zygotes. Alternative methods will be mentioned.

Keywords: CRISPR/Cas9; Electroporation; Genome editing; Mouse; Pronucleus injection.

MeSH terms

Animals
CRISPR-Cas Systems / genetics*
Electroporation
Gene Editing*
Mice
RNA, Guide, CRISPR-Cas Systems / genetics
Zygote / metabolism

Substances

RNA, Guide, CRISPR-Cas Systems