Enhanced nitrogen removal of the simultaneous partial nitrification, anammox and denitrification (SNAD) biofilm reactor for treating mainstream wastewater under low dissolved oxygen (DO) concentration

Zhaoming Zheng; Shan Huang; Wei Bian; Dongbo Liang; Xiujie Wang; Kai Zhang; Xiaoran Ma; Jun Li

doi:10.1016/j.biortech.2019.01.148

Enhanced nitrogen removal of the simultaneous partial nitrification, anammox and denitrification (SNAD) biofilm reactor for treating mainstream wastewater under low dissolved oxygen (DO) concentration

Bioresour Technol. 2019 Jul:283:213-220. doi: 10.1016/j.biortech.2019.01.148. Epub 2019 Feb 8.

Authors

Zhaoming Zheng¹, Shan Huang², Wei Bian¹, Dongbo Liang¹, Xiujie Wang¹, Kai Zhang¹, Xiaoran Ma¹, Jun Li³

Affiliations

¹ The College of Architecture and Civil Engineering, Beijing University of Technology, Beijing 100124, China.
² Department of Civil and Environmental Engineering, Princeton University, Princeton, NJ 08544, USA.
³ The College of Architecture and Civil Engineering, Beijing University of Technology, Beijing 100124, China. Electronic address: jglijun@bjut.edu.cn.

PMID: 30908986
DOI: 10.1016/j.biortech.2019.01.148

Abstract

The simultaneous partial nitrification, anammox and denitrification (SNAD) process for treating mainstream wastewater was investigated under different intermittent aeration modes. By controlling the aeration time of 20, 60 and 180 min during the intermittent modes, the oxygen concentration remained 3.50, 1.45 and 0.70 mg·L^-1. Correspondingly, the reactor achieved the nitrogen removal rate of 0.17, 0.29 and 0.30 kg N·m^-3·d^-1. Meanwhile, the average total inorganic nitrogen (TIN) removal efficiency reached 93.4%, 87.5% and 92.7%. The effluent NO₃^--N concentration was very low. High-throughput sequencing analysis indicated that the proportion of nitrite oxidization bacteria (NOB), anammox bacteria and denitrification bacteria was 0.15%, 0.33% and 8.78%. Candidatus Anammoxoglobus was the abundant anammox bacteria genus. Further study on the unclassified sequences revealed the possibility of the high relative abundance of Nitrosomonas-related genus and Candidatus Kuenenia-related genus on the SNAD biofilm.

Keywords: Biofilm reactor; Intermittent aeration; Low DO concentration; Low ammonium concentration; SNAD.

MeSH terms

Biofilms*
Denitrification
Nitrification
Nitrogen / metabolism*
Nitrosomonas / metabolism
Oxidation-Reduction
Oxygen / metabolism*
Wastewater / chemistry*

Substances

Waste Water
Nitrogen
Oxygen