In recent years, the determination of phenolic compounds in vegetable oil has aroused broad attention because these compounds have beneficial effects on health. In this work, a novel method based on the quick, easy, cheap, effective, rugged, and safe (QuEChERS) method and LC-MS/MS was developed for the analysis of phenolic compounds. A total of 18 mL of acetonitrile, 3 mL of water, and 270 mg of C18 sorbent were utilized in the optimized QuEChERS procedure. The LC-MS/MS analysis was performed in a C18 column under gradient-elution conditions with eluent of acetonitrile and water with 0.1% acetic acid. The QuEChERS approach achieved decent extraction recoveries (75.32-103.93%) for most phenolic compounds. The QuEChERS-LC-MS/MS method was validated in terms of accuracy, precision, sensitivity, and linearity. The proposed method was further evaluated using different prepared rapeseed oils. The result demonstrated that QuEChERS-LC-MS/MS is a rapid and reliable method for determining phenolic compounds in rapeseed oils.
Keywords: LC-MS/MS; QuEChERS; phenolic compounds; rapeseed oil.