Assessment of induced genomic instability in rat primary astrocytes exposed to intermediate frequency magnetic fields

Mikko Herrala; Jonne Naarala; Jukka Juutilainen

doi:10.1016/j.envres.2019.03.033

Assessment of induced genomic instability in rat primary astrocytes exposed to intermediate frequency magnetic fields

Environ Res. 2019 Jun:173:112-116. doi: 10.1016/j.envres.2019.03.033. Epub 2019 Mar 17.

Authors

Mikko Herrala¹, Jonne Naarala², Jukka Juutilainen²

Affiliations

¹ Department of Environmental and Biological Sciences, University of Eastern Finland, Kuopio, Finland. Electronic address: mikko.herrala@uef.fi.
² Department of Environmental and Biological Sciences, University of Eastern Finland, Kuopio, Finland.

PMID: 30903815
DOI: 10.1016/j.envres.2019.03.033

Abstract

We investigated whether exposure to intermediate frequency magnetic fields (IF MFs) could induce or enhance genomic instability in primary astrocytes. Rat primary astrocytes were exposed to vertical or horizontal 7.5 kHz, 300 μT MF for 24 h. To study possible combined effects with known genotoxic agents, the cells were exposed for 3 h to menadione or methyl methanesulfonate after the MF treatment. Induced genomic instability was evaluated 36 days after exposures using the Comet assay and flow cytometric scoring of micronuclei. Exposure to 7.5 kHz, 300 μT MF did not induce genomic instability alone or in combination with chemicals in measurements performed several cell generations after exposure.

Keywords: Comet assay; Induced genomic instability; Intermediate frequency; Magnetic fields; Micronucleus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Astrocytes*
Comet Assay
DNA Damage
Genomic Instability*
Magnetic Fields*
Micronucleus Tests
Rats