N-phosphonomethylglycine utilization by the psychrotolerant yeast Solicoccozyma terricola M 3.1.4

Bioorg Chem. 2019 Dec:93:102866. doi: 10.1016/j.bioorg.2019.03.040. Epub 2019 Mar 20.

Abstract

Solicoccozyma terricola M 3.1.4., the yeast strain isolated from soil sample from blueberry cultivation in Miedzyrzec Podlaski in Poland, is capable to split of phosphorus to nitrogen and nitrogen to carbon bonds in N-phosphonomethylglycine (PMG, glyphosate). The biodegradation process proceeds in the phosphate-independent manner. It is the first example of a psychrotolerant yeast strain able to degrade PMG via CN bond cleavage accompanied by AMPA formation and not like in most microorganisms via CP bond disruption followed by the sarcosine pathway. Glyphosate oxidoreductase (GOX) type activity was detected in cell-free extracts prepared from S. terricola M 3.1.4. pregrown on 4 mM PMG as a sole phosphorus and nitrogen source in cultivation medium.

Keywords: Glyphosate oxidoreductase; N-phosphonomethylglycine biodegradation; Phosphorus-nitrogen bond cleavage; Psychrotolerant yeasts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA, Fungal
  • Glycine / analogs & derivatives*
  • Glycine / chemistry
  • Glycine / metabolism*
  • Glyphosate
  • Organophosphonates / metabolism
  • Oxidoreductases / metabolism
  • Phosphorus / metabolism
  • Phylogeny
  • Yeasts / genetics
  • Yeasts / metabolism*

Substances

  • DNA, Fungal
  • Organophosphonates
  • Phosphorus
  • aminomethylphosphonic acid (AMPA)
  • Oxidoreductases
  • Glycine