Preparation, evaluation and application of core-shell molecularly imprinted particles as the sorbent in solid-phase extraction and analysis of lincomycin residue in pasteurized milk

Abstract

In this study, core-shell lincomycin-imprinted polymers were successfully synthesized and their binding properties evaluated. The functional monomers of methacrylamide and acrylamide were used for synthesis of core and shell, respectively. The optimum synthesized core-shell molecularly imprinted polymer (MIP) was applied as a sorbet in solid phase extraction cartridge. Afterwards, the method of core-shell molecularly imprinted solid phase extraction (CSMISPE) was used for pre-concentration and clean-up of lincomycin in the milk matrix prior to analysis via high performance liquid chromatography equipped with UV detector (HPLC-UV). The linear range for analysis of lincomycin in the milk matrix using introduced method was obtained from 0.08 to 2 μg/mL with recovery range of 80%-89%. The limit of detection and limit of quantification were 0.02 μg/mL and 0.08 μg/mL, respectively. Finally, calibrated CSMISPE-HPLC-UV method was used for lincomycin residue checking and quantification in the pasteurized milk samples of Mashhad city market.

Keywords: 2, 2′-Azobis-isobutyronitrile (PubChem CID: 6547); Acetonitrile (PubChem CID: 6342); Acrilamide (PubChem CID: 6579); Core-shell molecularly imprinted polymer; Dimethyl sulfoxide (PubChem CID: 679); Ethylene glycol dimethacrylate (PubChem CID: 7355); Hydrochloric acid (PubChem CID: 313); Lincomycin; Lincomycin hydrochloride (PubChem CID: 64710); Methacrylamide (PubChem CID: 6595); Methacrylic acid (PubChem CID: 4093); Methanol (PubChem CID: 887); Pasteurized milk; Solid phase extraction.