New micro-glass-tube leukocyte adherence inhibition assay assessing cell adherence of mononuclear cell subpopulations defined by monoclonal antibodies

J Immunol Methods. 1986 Jul 24;91(2):225-9. doi: 10.1016/0022-1759(86)90482-5.

Abstract

A new micro-glass-tube leukocyte adherence inhibition (LAI) assay which is appropriate for detecting delayed type hypersensitivity in vitro has been developed for human leukocytes. Enumeration of adherent cells is replaced by a cellular radioimmunoassay determining antibody binding of the monoclonal reagents, OKT4, OKT8 and OKM1, to glass-adherent cells, fixed by glutaraldehyde or formaldehyde. An LAI reactivity to purified protein derivative of tuberculin (PPD) was detectable in donors giving a positive PPD skin test with OKT4 reagent, but not with the other two reagents.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal*
  • Antigens, Differentiation, T-Lymphocyte
  • Antigens, Surface / analysis
  • Dose-Response Relationship, Immunologic
  • Humans
  • Immunologic Techniques*
  • Leukocyte Adherence Inhibition Test / methods*
  • Monocytes / classification
  • Monocytes / immunology*
  • Skin Tests
  • T-Lymphocytes / classification
  • T-Lymphocytes / immunology*
  • Tuberculin / immunology

Substances

  • Antibodies, Monoclonal
  • Antigens, Differentiation, T-Lymphocyte
  • Antigens, Surface
  • Tuberculin