First successful live birth following the use of MSOME and time lapse for sperm and embryo selections in a patient with severe male factor infertility: A case report

Esmat Mangoli; Mohammad Ali Khalili; Maryam Eftekhar; Guido Macchiarelli; Maria Grazia Palmerini

doi:10.1016/j.jogoh.2019.03.004

First successful live birth following the use of MSOME and time lapse for sperm and embryo selections in a patient with severe male factor infertility: A case report

J Gynecol Obstet Hum Reprod. 2019 Dec;48(10):883-885. doi: 10.1016/j.jogoh.2019.03.004. Epub 2019 Mar 18.

Authors

Esmat Mangoli¹, Mohammad Ali Khalili², Maryam Eftekhar³, Guido Macchiarelli⁴, Maria Grazia Palmerini⁴

Affiliations

¹ Department of Reproductive Biology, Yazd Institute for Reproductive Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
² Department of Reproductive Biology, Yazd Institute for Reproductive Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Electronic address: khalili59@hotmail.com.
³ Department of Gynecology, Yazd Institute for Reproductive Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
⁴ Department of Life, Health and Environmental Sciences, University of L'Aquila, L'Aquila, Italy.

PMID: 30898628
DOI: 10.1016/j.jogoh.2019.03.004

Abstract

We report a case of healthy live birth from a couple diagnosed with oligoasthenoteratozoospermia (OAT). They failed three ICSI cycles in the past 11 years. In the last cycle, the ovarian stimulation was done with antagonist protocol and six oocytes were aspirated. Semen sample was prepared by one layer gradient. 10 class I spermatozoa were selected by motile-sperm organelle-morphology examination (MSOME) according to Cassuto criteria. The selected spermatozoa were then injected into the MII oocytes. 16-18 h after ICSI, three zygotes was formed and, then, cultured in the time lapse monitoring (TLM) for 3 days. Two best embryos were selected according to the morphokinetic status for transfer. A singleton pregnancy was achieved, resulting in the birth of a healthy baby. This successful outcome shows that use of high technologies of MSOME and TLM procedures are useful for selection of the best spermatozoa and embryos in case of severe male infertility.

Keywords: Infertility; Live birth; MSOME; Sperm; Time lapse.

Publication types

Case Reports

MeSH terms

Adult
Female
Humans
Live Birth*
Male
Oligospermia*
Ovulation Induction / methods*
Semen Analysis / methods*
Single Embryo Transfer / methods
Sperm Injections, Intracytoplasmic / methods*
Sperm Motility
Sperm Retrieval
Time-Lapse Imaging / methods*