Evaluation of Serum Biochemical Parameters, Structural Changes, Immunohistochemistry and Parasite Load in the Urinary System of Dogs Infected Naturally by Leishmania infantum

J L Silva; V V G Oliveira; L A M T Silva; R P E Silva; L C Alves; M P Cavalcanti; V A Silva Júnior

doi:10.1016/j.jcpa.2018.11.007

Evaluation of Serum Biochemical Parameters, Structural Changes, Immunohistochemistry and Parasite Load in the Urinary System of Dogs Infected Naturally by Leishmania infantum

J Comp Pathol. 2019 Feb:167:26-31. doi: 10.1016/j.jcpa.2018.11.007. Epub 2019 Jan 7.

Authors

J L Silva¹, V V G Oliveira², L A M T Silva³, R P E Silva³, L C Alves⁴, M P Cavalcanti³, V A Silva Júnior⁵

Affiliations

¹ Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros s/n, Dois Irmãos, Recife.
² Centro Acadêmico de Vitória, Universidade Federal de Pernambuco, Rua Alto do Reservatório s/n, Bela Vista, Vitória de Santo Antão. Electronic address: vinicius-vasconcelos@hotmail.com.
³ Fundação Oswaldo Cruz (Fiocruz) - Pernambuco, Centro de Pesquisas Aggeu Magalhães, Campus da Universidade Federal de Pernambuco, Cidade Universitária, Av. Moraes Rego, s/n, Recife.
⁴ Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros s/n, Dois Irmãos, Recife, Brazil.
⁵ Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros s/n, Dois Irmãos, Recife, Brazil. Electronic address: vinicius-vasconcelos@hotmail.com.

PMID: 30898294
DOI: 10.1016/j.jcpa.2018.11.007

Abstract

The aim of this study was to evaluate the histopathological changes in the urinary system and their correlation with serum biochemical parameters, parasite load and immunodetection of amastigotes in 30 dogs infected naturally by Leishmania infantum. The major histological changes were glomerulonephritis and interstitial nephritis. Serum biochemistry demonstrated that the dogs had progressive loss of renal function. L. infantum amastigotes were detected immunohistochemically in all parts of the urinary system, except the ureters. There was a correlation between parasite load, severity of histopathological changes and immunodetection of the parasites, but parasite load was not correlated with the serum biochemical parameters evaluated.

Keywords: dog; immunohistochemistry; urinary system; visceral leishmaniosis.

MeSH terms

Animals
Dog Diseases / metabolism
Dog Diseases / parasitology*
Dog Diseases / pathology*
Dogs
Immunohistochemistry
Leishmania infantum
Leishmaniasis, Visceral / veterinary*
Male
Parasite Load
Urinary Tract Infections / veterinary*