Multiple studies have examined the impacts of various nuts on appetitive and metabolic effects; however, fewer studies have addressed the impacts of mixed nuts. The objective was to examine the acute effects of consumption of an isocaloric (253 kcal) snack of mixed nuts or pretzels on appetite hormones, glucose and insulin responses, and subjective appetite ratings in overweight and obese adults. In a two-arm randomized controlled trial, overweight and obese adults consumed either mixed nuts (n = 27) or pretzels (n = 27) with 16 men and 11 women per group. After an overnight fast, blood glucose, insulin, and appetite hormone were measured at baseline and 60 min post snack consumption. Appetite/satiety questionnaires were completed at baseline and 20, 40, 60, 90, 120 min after snack consumption. Both snacks increased satiety and reduced hunger over time. The pretzels group exhibited higher subjective satiety and lower hunger (P < .001) following consumption than the mixed nuts group. Pretzel consumption increased glucose and insulin (P < .001), while no elevation was detected in the mixed nuts at 60 min post snack consumption. Leptin and ghrelin concentrations were significantly lower for the mixed nuts group at 60 min compared with baseline (P < .05). Mixed nuts promote satiety in overweight and obese adults while maintaining stable blood glucose and insulin levels. These results suggest that mixed nuts snack may be beneficial for those who are overweight or obese. Future mixed nuts studies are warranted to examine long-term effects.