Objectives: To study the decomposition kinetics of omethoate in blood.
Methods: The acetonitrile precipitated protein was added into the blood, with the chromatographic column of a Waters BEH C18 column (2.1 mm×50 mm, 1.7 μm), the mobile phase of 5 mmol/L ammonium acetate aqueous solution-methanol, and the gradient elution with a flow rate of 0.3 mL/min and injection volume of 2 μL. With electrospray ionization (ESI) source and positive ion detection, qualitative and quantitative analyses were taken using multi-reaction monitoring mode. Omethoate standard was added into blank human blood to the mass concentrations of 0.78, 1.40, 2.30, 4.50, and 7.20 μg/mL, and each mass concentration was preserved at 3 temperatures of -20 ℃, 4 ℃, and 20 ℃, respectively. The content of omethoate was detected at different time points (0, 1, 3, 4, 7, 11, 15, 24, 32, 40, 48, 64, 80, 96, and 120 d).
Results: Different concentrations of omethoate all showed a descended trend in human blood under different temperature conditions. The decomposition in storage environment of -20 ℃, 4 ℃, and 20 ℃ was fit to a one-compartment open model with a first-order kinetic process, which could be expressed as Ct=Coe-αt, with the calculated theoretical values of omethoate concentration close to the measured values.
Conclusions: All concentrations of omethoate are decomposed in the blood, which vary a lot in different preservation conditions. It is suggested that blood samples should be frozen and detected timely in suspected omethoate poisoning cases.
题目: 氧化乐果在血液中的分解动力学.
目的: 研究氧化乐果在血液中的分解动力学变化规律。.
方法: 血液中加入乙腈沉淀蛋白,色谱柱采用Waters BEH C18柱(2.1 mm×50 mm,1.7 μm),流动相为5 mmol/L乙酸铵水溶液-甲醇,梯度洗脱,流速为0.3 mL/min,进样量为2 μL;电喷雾离子(electrospray ionization,ESI)源,正离子检测,采用多反应监测模式进行定性定量分析。取空白人血液分别添加氧化乐果标准品至质量浓度为0.78、1.40、2.30、4.50、7.20 μg/mL,每种质量浓度均分别置于-20 ℃、4 ℃、20 ℃三种温度条件下保存,于不同时间点(0、1、3、4、7、11、15、24、32、40、48、64、80、96和120 d)检测其中氧化乐果的含量。.
结果: 在不同温度环境下,不同质量浓度的氧化乐果在人血液中均呈下降趋势,在-20 ℃、4 ℃、20 ℃保存环境下分解符合一级动力学过程一室开放模型,可以采用Ct=Coe-αt表示,根据Ct=Coe-αt计算的各样品中氧化乐果含量理论值与实测值接近。.
结论: 不同质量浓度的氧化乐果在血液中均存在分解现象,且在不同保存环境条件下存在很大差异,实际检案中对疑似氧化乐果中毒的案件,提取检材后应冷冻保存并及时送检。.
关键词: 法医毒理学;液相色谱-质谱法;氧化乐果;血液.
Keywords: forensic toxicology; liquid chromatography-mass spectrometry; omethoate; blood.
Copyright© by the Editorial Department of Journal of Forensic Medicine.