Abstract
The extracellular SspA protease of the human pathogen S. aureus is essential for evading the host immune defence system. Two novel FRET probes for selective real-time ratiometric imaging of SspA activity in live S. aureus cells are reported.
MeSH terms
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Anti-Bacterial Agents / chemical synthesis
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Anti-Bacterial Agents / chemistry
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Anti-Bacterial Agents / pharmacology
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Bacterial Proteins
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Carbocyanines / chemical synthesis
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Carbocyanines / chemistry*
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Carbocyanines / pharmacology
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Cell Membrane / chemistry
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Cellular Microenvironment
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Enzyme Assays / methods
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Fluorescence Resonance Energy Transfer / methods
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Fluorescent Dyes / chemical synthesis
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Fluorescent Dyes / chemistry*
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Fluorescent Dyes / pharmacology
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Indoles / chemical synthesis
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Indoles / chemistry*
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Indoles / pharmacology
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Microbial Sensitivity Tests
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Microscopy, Confocal / methods
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Microscopy, Fluorescence / methods
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Oligopeptides / chemical synthesis
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Oligopeptides / chemistry*
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Oligopeptides / pharmacology
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Palmitates / chemical synthesis
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Palmitates / chemistry
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Palmitates / pharmacology
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Proteolysis
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Sensitivity and Specificity
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Serine Endopeptidases / analysis*
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Staphylococcus aureus / drug effects
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Staphylococcus aureus / enzymology
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Carbocyanines
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Fluorescent Dyes
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Indoles
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Oligopeptides
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Palmitates
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Serine Endopeptidases
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glutamyl endopeptidase