Ratiometric fluorescent probes for selective and sensitive visualization of bacterial microenvironment protease activity

Chem Commun (Camb). 2019 Apr 25;55(35):5064-5067. doi: 10.1039/c9cc00953a.

Abstract

The extracellular SspA protease of the human pathogen S. aureus is essential for evading the host immune defence system. Two novel FRET probes for selective real-time ratiometric imaging of SspA activity in live S. aureus cells are reported.

MeSH terms

  • Anti-Bacterial Agents / chemical synthesis
  • Anti-Bacterial Agents / chemistry
  • Anti-Bacterial Agents / pharmacology
  • Bacterial Proteins
  • Carbocyanines / chemical synthesis
  • Carbocyanines / chemistry*
  • Carbocyanines / pharmacology
  • Cell Membrane / chemistry
  • Cellular Microenvironment
  • Enzyme Assays / methods
  • Fluorescence Resonance Energy Transfer / methods
  • Fluorescent Dyes / chemical synthesis
  • Fluorescent Dyes / chemistry*
  • Fluorescent Dyes / pharmacology
  • Indoles / chemical synthesis
  • Indoles / chemistry*
  • Indoles / pharmacology
  • Microbial Sensitivity Tests
  • Microscopy, Confocal / methods
  • Microscopy, Fluorescence / methods
  • Oligopeptides / chemical synthesis
  • Oligopeptides / chemistry*
  • Oligopeptides / pharmacology
  • Palmitates / chemical synthesis
  • Palmitates / chemistry
  • Palmitates / pharmacology
  • Proteolysis
  • Sensitivity and Specificity
  • Serine Endopeptidases / analysis*
  • Staphylococcus aureus / drug effects
  • Staphylococcus aureus / enzymology

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Carbocyanines
  • Fluorescent Dyes
  • Indoles
  • Oligopeptides
  • Palmitates
  • Serine Endopeptidases
  • glutamyl endopeptidase