The BARA necessities of PtdIns 3-kinase activation in autophagy

Chunmei Chang; Lindsey N Young; James H Hurley

doi:10.1080/15548627.2019.1596501

The BARA necessities of PtdIns 3-kinase activation in autophagy

Autophagy. 2019 Jun;15(6):1122-1123. doi: 10.1080/15548627.2019.1596501. Epub 2019 Mar 30.

Authors

Chunmei Chang^{1

2}, Lindsey N Young^{1

2

3}, James H Hurley^{1

2

3}

Affiliations

¹ a Department of Molecular and Cell Biology , University of California , Berkeley , CA , USA.
² b California Institute for Quantitative Biosciences , University of California , Berkeley , CA , USA.
³ c Molecular Biophysics and Integrated Bioimaging Division , Lawrence Berkeley National Laboratory , Berkeley , CA , USA.

Abstract

Macroautophagy/autophagy is an evolutionarily conserved degradation system with fundamental biological functions. The activation of the class III phosphatidylinositol 3-kinase (PtdIns3K) complexes and the subsequent production of phosphatidylinositol 3-phosphate (PtdIns3P) are pivotal to autophagy. Using a combination of structural biology, biochemistry, and biophysics, we revealed how the non-catalytic subunit BECN1 serves as a membrane-binding switch in the regulation of PtdIns3K complexes and autophagy.

Keywords: Cryoelectron microscopy; HIV; Nef; Rubicon; Tat-BECN1 peptide; human immunodeficiency virus; hydrogen-deuterium exchange.

Publication types

Research Support, N.I.H., Extramural
Comment

MeSH terms

Autophagy*
Class III Phosphatidylinositol 3-Kinases

Substances

Class III Phosphatidylinositol 3-Kinases

Abstract

Publication types

MeSH terms

Substances

Grants and funding