Inhibition of lipid accumulation by the ethyl acetate fraction of Distylium racemosum in vitro and in vivo

Hye-Ran Kim; Bo-Kyoung Jung; Min-Ho Yeo; Weon-Jong Yoon; Kyung-Soo Chang

doi:10.1016/j.toxrep.2019.02.003

Inhibition of lipid accumulation by the ethyl acetate fraction of Distylium racemosum in vitro and in vivo

Toxicol Rep. 2019 Feb 23:6:215-221. doi: 10.1016/j.toxrep.2019.02.003. eCollection 2019.

Authors

Hye-Ran Kim^{1

2}, Bo-Kyoung Jung¹, Min-Ho Yeo¹, Weon-Jong Yoon³, Kyung-Soo Chang¹

Affiliations

¹ Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan, Busan 46252, Republic of Korea.
² Department of Clinical Laboratory Science, College of Medical Sciences, Daegu Haany University, Gyeongsan 38610, Republic of Korea.
³ Jeju Biodiversity Research Institute, Jeju Technopark, Jeju 63208, Republic of Korea.

Abstract

This study confirms the anti-obesity effect of the ethyl acetate fraction of Distylium racemosum (DRE), a member of Hamamelidaceae, that naturally grows on Jeju Island, on adipocyte differentiation in 3T3-L1 cells. This study further demonstrated that DRE exhibits anti-obesity effects in C57BL/6 obese mice. The degree of adipocyte differentiation was determined using Oil red O stain; results indicated a decrease in fat globules, which was dependent on DRE concentration, when pre-adipocytes were treated with differentiation-inducing agents. In addition, this significantly reduced the expression of the adipogenic transcription factor and related genes. C57BL/6 obese mice treated with DRE showed a lower rate of body weight gain than the high-fat diet (HFD) group mice. Further, the level of serum triglyceride in the DRE treatment group was lower than that in the HFD group. The findings show that DRE are capable of suppressing adipocyte accumulation; therefore, DRE may represent a promising source of functional materials for the anti-obesity.

Keywords: Distylium racemosum; Ethyl acetate fraction; Obesity.