Biological relevance of CuFeO2 nanoparticles: Antibacterial and anti-inflammatory activity, genotoxicity, DNA and protein interactions

Abstract

Heterometal oxide nanoparticles of bioessential metals are shedding new light to nanoparticle-inspired bioapplications. Pairing bioreactive elements like copper and iron can affect the redox dynamic and biological profile of the nanomaterial. Given the complexity of physicochemical properties, biological activity and toxicity concerns, extensive exploration is demanded, especially when active and less active oxidation states participate as in case of cuprous-ferric delafossite CuFeO₂ (copper(I)-iron(III)), a less widespread nanomaterial. In that vein, CuFeO₂ nanoparticles were synthesized and biological profile was evaluated in comparison with cuprous oxide (Cu₂O NPs) counterpart, an already established antimicrobial agent. Interactions with bacteria, proteins and DNA were examined. Cu₂O NPs exhibited stronger antibacterial activity (IC₅₀ < 25 μg/ml) than CuFeO₂ NPs (IC₅₀ > 100 μg/ml). In vitro exposure of nanoparticles on plasmid DNA unveiled toxicity in the form of DNA damage for Cu₂O and enhanced biocompatibility for CuFeO₂ NPs. Genotoxicity estimated by the frequency of sister chromatid exchanges, cytostaticity based on the proliferating rate indices and cytotoxicity based on the mitotic indices at human peripheral lymphocyte cultures were all significantly lower in the case of CuFeO₂ NPs. Furthermore, through in vitro albumin denaturation assay, CuFeO₂ NPs showed better performance in protein denaturation protection, correlating in superior anti-inflammatory activity than Cu₂O and similar to acetylsalicylic acid. Synergy of copper(I)-iron(III) in nanoscale is apparent and gives rise to fruitful bioapplications and perspectives.

Keywords: Copper(I)-iron(III); Delafossite; Gel electrophoresis; Heterometal oxides; Sister chromatid exchange.