Molecular detection and genetic diversity of Babesia canis canis in pet dogs in Henan Province, China

Jinming Wang; Junlong Liu; Jifei Yang; Zhijie Liu; Xiaoxing Wang; Youquan Li; Jianxun Luo; Guiquan Guan; Hong Yin

doi:10.1016/j.parint.2019.03.011

Molecular detection and genetic diversity of Babesia canis canis in pet dogs in Henan Province, China

Parasitol Int. 2019 Aug:71:37-40. doi: 10.1016/j.parint.2019.03.011. Epub 2019 Mar 15.

Authors

Jinming Wang¹, Junlong Liu², Jifei Yang³, Zhijie Liu⁴, Xiaoxing Wang⁵, Youquan Li⁶, Jianxun Luo⁷, Guiquan Guan⁸, Hong Yin⁹

Affiliations

¹ State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu 730046, China. Electronic address: wjm0403@caas.cn.
² State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu 730046, China. Electronic address: liujunlong@caas.cn.
³ State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu 730046, China. Electronic address: yangjifei@caas.cn.
⁴ State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu 730046, China. Electronic address: liuzhijie@caas.cn.
⁵ State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu 730046, China.
⁶ State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu 730046, China. Electronic address: liyouquan@caas.cn.
⁷ State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu 730046, China. Electronic address: luojianxun@caas.cn.
⁸ State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu 730046, China. Electronic address: guanguiquan@caas.cn.
⁹ State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu 730046, China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis, Yangzhou University, Yangzhou 225009, China. Electronic address: yinhong@caas.cn.

PMID: 30885796
DOI: 10.1016/j.parint.2019.03.011

Abstract

Canine babesiosis is an important tick-borne disease caused by Babesia spp. A total of 130 blood samples were randomly collected from pet dogs in Zhengzhou city, Henan Province, China, and screened for the presence of piroplasms with nested PCR and gene sequencing targeting the 18S rRNA gene. On the basis of blast analysis of the 18S rRNA gene sequences, our results revealed that seven dogs (5.4%) were infected with Babesia canis canis. The sequences were compared with those in GenBank, and alignments showed that all B. canis canis isolates belonged to genotype B. This is the first report of B. canis canis infection in dogs in China.

Keywords: Babesia canis canis; Canine babesiosis; China; Pet dog.

MeSH terms

Animals
Babesia / genetics*
Babesia / isolation & purification
Babesiosis / blood
Babesiosis / diagnosis
China
DNA, Protozoan / genetics
Dog Diseases / diagnosis*
Dog Diseases / parasitology
Dogs / parasitology*
Female
Genetic Variation*
Genotype
High-Throughput Nucleotide Sequencing
Male
Pets / parasitology
Polymerase Chain Reaction
RNA, Ribosomal, 18S / genetics

Substances

DNA, Protozoan
RNA, Ribosomal, 18S