Identification of a novel lineage of plasmids within phylogenetically diverse subclades of IncHI2-ST1 plasmids

Piklu Roy Chowdhury; Mathieu Fourment; Matthew Z DeMaere; Leigh Monahan; John Merlino; Thomas Gottlieb; Aaron E Darling; Steven P Djordjevic

doi:10.1016/j.plasmid.2019.03.002

Identification of a novel lineage of plasmids within phylogenetically diverse subclades of IncHI2-ST1 plasmids

Plasmid. 2019 Mar:102:56-61. doi: 10.1016/j.plasmid.2019.03.002. Epub 2019 Mar 15.

Authors

Piklu Roy Chowdhury¹, Mathieu Fourment², Matthew Z DeMaere², Leigh Monahan², John Merlino³, Thomas Gottlieb³, Aaron E Darling², Steven P Djordjevic²

Affiliations

¹ The Ithree Institute, University of Technology Sydney, City Campus, Ultimo, NSW 2007, Australia; NSW Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, Sydney, Australia. Electronic address: Piklu.Bhattacharya@uts.edu.au.
² The Ithree Institute, University of Technology Sydney, City Campus, Ultimo, NSW 2007, Australia.
³ Department of Microbiology and Infectious Diseases, Concord Hospital, NSW Health Pathology, Hospital Road, Concord 2139, NSW, Australia; Faculty of Medicine, University of Sydney, NSW, Australia.

PMID: 30885788
DOI: 10.1016/j.plasmid.2019.03.002

Abstract

IncHI2-ST1 plasmids play an important role in co-mobilizing genes conferring resistance to critically important antibiotics and heavy metals. Here we present the identification and analysis of IncHI2-ST1 plasmid pSPRC-Echo1, isolated from an Enterobacter hormaechei strain from a Sydney hospital, which predates other multi-drug resistant IncHI2-ST1 plasmids reported from Australia. Our time-resolved phylogeny analysis indicates pSPRC-Echo1 represents a new lineage of IncHI2-ST1 plasmids and show how their diversification relates to the era of antibiotics.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromosome Mapping
DNA Transposable Elements / genetics
Phylogeny*
Plasmids / genetics*
Time Factors

Substances

DNA Transposable Elements