MiR-223 promotes oral squamous cell carcinoma proliferation and migration by regulating FBXW7

Cancer Biomark. 2019;24(3):325-334. doi: 10.3233/CBM-181877.

Abstract

Abnormally expressed microRNAs (miRNAs) contribute widely to human cancer, including oral squamous cell carcinoma (OSCC), by regulating their downstream targets. MiR-223 has been proved to be up-regulated in both gastric cancer and ovarian cancer. However, the effect of miR-223 on OSCC is still unclear. Here, we showed that miR-223 was over-expressed in OSCC tissues using qRT-PCR. Next, we investigated the biological mechanism of miR-223 in OSCC. The results demonstrated that miR-223 facilitated the cell proliferation and migration of OSCC using MTT assay and Transwell assay. Furthermore, we stated that the FBXW7 expression was decreased in OSCC and re-expression of FBXW7 inhibited the proliferation and migration of OSCC. In addition, FBXW7 mimic inversed the promotion effect of miR-223 in regulating of OSCC cells. In short, miR-223 promoted OSCC cell proliferation and migration by downregulating FBXW7, which provided a novel therapeutic strategy for OSCC.

Keywords: FBXW7; MiR-223; OSCC; migration; proliferation.

MeSH terms

  • 3' Untranslated Regions
  • Adult
  • Aged
  • Apoptosis / genetics
  • Carcinoma, Squamous Cell / genetics*
  • Carcinoma, Squamous Cell / pathology
  • Cell Line, Tumor
  • Cell Movement / genetics
  • Cell Proliferation
  • Disease Progression
  • F-Box-WD Repeat-Containing Protein 7 / genetics*
  • Female
  • Gene Expression Regulation, Neoplastic*
  • Genes, Reporter
  • Humans
  • Male
  • MicroRNAs / genetics*
  • Middle Aged
  • Mouth Neoplasms / genetics*
  • Mouth Neoplasms / pathology
  • Neoplasm Staging
  • RNA Interference*
  • Tumor Burden

Substances

  • 3' Untranslated Regions
  • F-Box-WD Repeat-Containing Protein 7
  • FBXW7 protein, human
  • MIRN223 microRNA, human
  • MicroRNAs