A polysaccharide isolated and purified from Platycladus orientalis (L.) Franco leaves, characterization, bioactivity and its regulation on macrophage polarization

Jiaoyan Ren; Chuanli Hou; Chuanchao Shi; Zehua Lin; Wenzhen Liao; Erdong Yuan

doi:10.1016/j.carbpol.2019.03.003

A polysaccharide isolated and purified from Platycladus orientalis (L.) Franco leaves, characterization, bioactivity and its regulation on macrophage polarization

Carbohydr Polym. 2019 Jun 1:213:276-285. doi: 10.1016/j.carbpol.2019.03.003. Epub 2019 Mar 4.

Authors

Jiaoyan Ren¹, Chuanli Hou², Chuanchao Shi², Zehua Lin², Wenzhen Liao³, Erdong Yuan⁴

Affiliations

¹ School of Food Science and Engineering, South China University of Technology, Guangzhou, 510641, China; Sino-Singapore International Joint Research Institute, Guangzhou Knowledge City, Huangpu District, Guangzhou, 510663, China.
² School of Food Science and Engineering, South China University of Technology, Guangzhou, 510641, China.
³ Department of Nutrition and Food Hygiene, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, 510515, Guangdong, China. Electronic address: wenzhenliao@163.com.
⁴ School of Food Science and Engineering, South China University of Technology, Guangzhou, 510641, China. Electronic address: erdyuan@scut.edu.cn.

PMID: 30879670
DOI: 10.1016/j.carbpol.2019.03.003

Abstract

The structure and bioactivity of a novel polysaccharide from Platycladus orientalis (L.) Franco leaves (POP2) were investigated in the present study. Structure characterization demonstrated that the average molecular weight of POP2 was 9.69 kDa and consisted of arabinose (14.39%), mannose (10.24%), glucose (63.95%) and galactose (11.42%). The main linkage types of POP2 consisted of (1→4)-linked α-d-Glc and (1→6)-linked α-d-Glc based on methylation and NMR analysis. Bioactivity evaluation showed that POP2 could effectively promote the secretion of inflammatory cytokines (IL-6 and TNF-α), as well as the anti-inflammatory cytokines (IL-10) in LPS-induced cells. Besides, the secretion of NO was significantly inhibited by POP2 in M1 model. POP2 could enhance the level of inflammatory cytokines (NO, IL-6 and TNF-α), while the secretion of the anti-inflammatory cytokine TGF-β was markedly suppressed in IL-4 induced cells. Our work attempted to elucidate the regulation of macrophage polarization and support the potential application of POP2 as bioactive ingredient for functional foods.

Keywords: Anti-inflammatory cytokines; Inflammatory cytokines; Macrophage polarization; Platycladus orientalis (L.) Franco leaves; RAW 264.7.

MeSH terms

Animals
Cell Polarity / drug effects
Cells, Cultured
Cupressaceae / chemistry*
Macrophages / drug effects*
Macrophages / metabolism
Mice
Plant Leaves / chemistry
Polysaccharides / chemistry
Polysaccharides / isolation & purification
Polysaccharides / pharmacology*
RAW 264.7 Cells

Substances

Polysaccharides