miR-500 promotes cell proliferation by directly targetting LRP1B in prostate cancer

Biosci Rep. 2019 Apr 5;39(4):BSR20181854. doi: 10.1042/BSR20181854. Print 2019 Apr 30.

Abstract

Accumulating evidence suggests that miRNAs play a crucial role in the development of prostate cancer (PC); however, the role of miR-500 in PC remains poorly understood. The data presented here reveal abnormal increases in miR-500 expression in PC tissues and cell lines. Suppression of miR-500 expression significantly inhibited the proliferation of PC-3 and LnCap cells and was negatively regulative with low-density lipoprotein receptor-related protein 1B (LRP1B). Increased cell cycle arrest at the G1 stage and decreased protein expression of cyclinD1 and CDK2 was observed in response to miR-500 knockdown in PC-3 and LnCap cells, in combination with LRP1B overexpression. LRP1B was identified as a target of miR-500 and was significantly decreased in PC tissues. Taken together, these findings demonstrate that miR-500 plays an important role in the proliferation of PC cells via the inhibition of LRP1B expression.

Keywords: LRP1B; miR-500; proliferation; prostate cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Cell Proliferation / genetics*
  • Cyclin D1 / biosynthesis
  • Cyclin-Dependent Kinase 2 / biosynthesis
  • G1 Phase Cell Cycle Checkpoints / genetics
  • Gene Expression Regulation, Neoplastic / genetics*
  • Gene Knockdown Techniques
  • Humans
  • Male
  • MicroRNAs / genetics*
  • PC-3 Cells
  • Prostatic Neoplasms / genetics*
  • Prostatic Neoplasms / pathology
  • Receptors, LDL / biosynthesis
  • Receptors, LDL / genetics*

Substances

  • CCND1 protein, human
  • LRP1B protein, human
  • MIRN500 microRNA, human
  • MicroRNAs
  • Receptors, LDL
  • Cyclin D1
  • CDK2 protein, human
  • Cyclin-Dependent Kinase 2