Long-term bond strength and endogenous enzymatic activity of a chlorhexidine-containing commercially available adhesive

Tatjana Maravić; Allegra Comba; Sandra Ribeiro Cunha; Valeria Angeloni; Milena Cadenaro; Erika Visinitini; Chiara Ottavia Navarra; Stefano Salgarello; Lorenzo Breschi; Annalisa Mazzoni

doi:10.1016/j.jdent.2019.03.004

Long-term bond strength and endogenous enzymatic activity of a chlorhexidine-containing commercially available adhesive

J Dent. 2019 May:84:60-66. doi: 10.1016/j.jdent.2019.03.004. Epub 2019 Mar 12.

Authors

Affiliations

¹ Department of Biomedical and Neuromotor Sciences, DIBINEM, University of Bologna - Alma Mater Studiorum, Via San Vitale 59, 40125, Bologna, Italy. Electronic address: tatjana.maravic2@unibo.it.
² Department of Biomedical and Neuromotor Sciences, DIBINEM, University of Bologna - Alma Mater Studiorum, Via San Vitale 59, 40125, Bologna, Italy. Electronic address: allegra.comba@unibo.it.
³ Department of Restorative Dentistry, School of Dentistry, University of São Paulo, Av. Prof. Lineu Prestes 2227, 05508-000, São Paulo, Brazil. Electronic address: sandra.cunha@gmail.com.
⁴ Private Practice, Via Martiri della Foce 20, 17031, Albenga, Italy. Electronic address: angeloni.valeria@gmail.com.
⁵ Department of Medical Sciences, University of Trieste, Piazza dell'Ospitale 1, 34129, Trieste, Italy. Electronic address: mcadenaro@units.it.
⁶ S.C. Clinica di Chirurgia Maxillofacciale e Odontostomatologia AsuiTS, Piazza dell'Ospitale 1, 34129, Trieste, Italy. Electronic address: E.VISINTINI@fmc.units.it.
⁷ Department of Medical, Surgical and Health Sciences, University of Trieste, Piazza dell'Ospitale 1, 34129, Trieste, Italy. Electronic address: c.navarra@fmc.units.it.
⁸ Dipartimento di Specialità Medico-Chirurgiche, Scienze Radiologiche e Sanità Pubblica - Università degli Studi di Brescia, P.le Spedali Civili 1, 25123, Brescia, Italy. Electronic address: stefano.salgarello@unibs.it.
⁹ Department of Biomedical and Neuromotor Sciences, DIBINEM, University of Bologna - Alma Mater Studiorum, Via San Vitale 59, 40125, Bologna, Italy. Electronic address: lorenzo.breschi@unibo.it.
¹⁰ Department of Biomedical and Neuromotor Sciences, DIBINEM, University of Bologna - Alma Mater Studiorum, Via San Vitale 59, 40125, Bologna, Italy. Electronic address: annalisa.mazzoni@unibo.it.

PMID: 30876948
DOI: 10.1016/j.jdent.2019.03.004

Abstract

Objectives: The aim of this study was to investigate, by the means of microtensile bond strength (μTBS) test, gelatin and in situ zymography, the influence of 0.2% CHX contained within a commercially available adhesive on long-term bond strength and endogenous enzymatic activity.

Methods: Non-carious teeth were subjected to μTBS test (N = 15 for each group) and stressed until failure. μTBS was evaluated immediately and after 12-month storage in artificial saliva at 37 °C. Dentin powder was obtained from additional teeth (N = 7) for gelatin zymography, while for in situ zymography, 3 teeth for each group were selected. Gelatin and in situ zymography were performed in dentin powder and slices of dentin, respectively, to assess the ability of 0.2% CHX blended within the adhesive to inhibit endogenous enzymatic activity.

Results: μTBS bond strength was higher in the CHX-containing groups, immediately as well as after aging. The bond strength significantly decreased after 12-month aging. The activation of endogenous MMPs was found to be related to the presence of CHX within the adhesive system and the bonding strategy employed.

Conclusions: Under this perspective 0.2% CHX blended within Peak Universal adhesive monomer seems to increase immediate bond strength, to preserve bond strength over time and to efficiently inhibit endogenous enzymatic activity in dentin. Hence, blending the CHX in low concentrations within the adhesive could be recommended as a feasible technique in every-day clinical practice.

Clinical significance: Using CHX-containing adhesives could be recommended due to several benefits: it seems to increase the longevity of the hybrid layer; the inhibitor appears to be efficiently delivered to the dentinal substrate and to inhibit endogenous enzymatic activity, without prolonging chair time.

Keywords: Aging; Chlorhexidine; Dental bonding systems; Dentin; Hybrid Layer.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chlorhexidine*
Dental Bonding*
Dental Cements
Dental Stress Analysis
Dentin
Dentin-Bonding Agents*
Materials Testing
Resin Cements
Tensile Strength

Substances

Dental Cements
Dentin-Bonding Agents
Resin Cements
Chlorhexidine