Quantifying both ammonium and proline in wines and beer by using a PDMS composite for sensoring

María Carmen Prieto-Blanco; Neus Jornet-Martinez; Jorge Verdú-Andrés; Carmen Molíns-Legua; Pilar Campíns-Falcó

doi:10.1016/j.talanta.2019.02.001

Quantifying both ammonium and proline in wines and beer by using a PDMS composite for sensoring

Talanta. 2019 Jun 1:198:371-376. doi: 10.1016/j.talanta.2019.02.001. Epub 2019 Feb 4.

Authors

María Carmen Prieto-Blanco¹, Neus Jornet-Martinez², Jorge Verdú-Andrés², Carmen Molíns-Legua², Pilar Campíns-Falcó³

Affiliations

¹ Universidade da Coruña, Grupo QANAP, Instituto Universitario de Medio Ambiente (IUMA), Centro de Investigacións Científicas Avanzadas (CICA), Departamento de Química Analítica, Facultade de Ciencias, Zapateira, 15071 A Coruña, Spain. Electronic address: mcprieto@udc.es.
² Grupo MINTOTA, Departament de Química Analítica, Facultat de Química, Universitat de Valencia, C/ Dr. Moliner 50, E46100 Burjassot, Valencia, Spain.
³ Grupo MINTOTA, Departament de Química Analítica, Facultat de Química, Universitat de Valencia, C/ Dr. Moliner 50, E46100 Burjassot, Valencia, Spain. Electronic address: pilar.campins@uv.es.

PMID: 30876574
DOI: 10.1016/j.talanta.2019.02.001

Abstract

Two of the reagents involved in the Berthelot's reaction, thymol and nitroprusside, were embedded in a PDMS composite in order to apply this assay to determine ammonium and proline, in wine and beers. Safety, portability, rapidity, cost-effectiveness and simplicity of the assay were improved. For the proline determination, a modified Berthelot's reaction, which included a ring cleavage of proline, was optimized. The accuracy of the assay was tested. The limits of detection for ammonium was 0.12 µg mL^-1 and for proline was in the range from 0.7 to 4.1 µg mL^-1, depending on the kind of wine (white, red, or sweet), for beer the LOD was 6 µg mL^-1. The precision achieved was slower than 10%. The accuracy of the assay was tested by means of a confirmatory validation study. Good results were obtained for real samples.

Keywords: Ammonium ion; Colorimetric sensor; Double solid device; PDMS composite; Proline; Wines.