An efficient new dual fluorescent pyrene based chemosensor for the detection of bismuth (III) and aluminium (III) ions and its applications in bio-imaging

Arjunan Saravanan; Selvaraj Shyamsivappan; Thangaraj Suresh; Gopalan Subashini; Krishna Kadirvelu; Nanjan Bhuvanesh; Raju Nandhakumar; Palathurai Subramaniam Mohan

doi:10.1016/j.talanta.2019.01.114

An efficient new dual fluorescent pyrene based chemosensor for the detection of bismuth (III) and aluminium (III) ions and its applications in bio-imaging

Talanta. 2019 Jun 1:198:249-256. doi: 10.1016/j.talanta.2019.01.114. Epub 2019 Feb 1.

Authors

Arjunan Saravanan¹, Selvaraj Shyamsivappan², Thangaraj Suresh², Gopalan Subashini³, Krishna Kadirvelu⁴, Nanjan Bhuvanesh⁵, Raju Nandhakumar⁶, Palathurai Subramaniam Mohan⁷

Affiliations

¹ Department of Chemistry, Bharathiar University, Coimbatore 641046, Tamil Nadu, India; BU-DRDO CLS, Bharathiar University Campus, Coimbatore 641046, Tamil Nadu, India.
² Department of Chemistry, Bharathiar University, Coimbatore 641046, Tamil Nadu, India.
³ Department of Chemistry, P.S.G.R. Krishnammal College for Women, Coimbatore 641004, Tamil Nadu, India.
⁴ BU-DRDO CLS, Bharathiar University Campus, Coimbatore 641046, Tamil Nadu, India.
⁵ Department of Chemistry, Karunya Institute of Technology and Sciences, Coimbatore 641114, Tamil Nadu, India.
⁶ Department of Chemistry, Karunya Institute of Technology and Sciences, Coimbatore 641114, Tamil Nadu, India. Electronic address: nandhakumar@karunya.edu.
⁷ Department of Chemistry, Bharathiar University, Coimbatore 641046, Tamil Nadu, India. Electronic address: psmohan59@gmail.com.

PMID: 30876558
DOI: 10.1016/j.talanta.2019.01.114

Abstract

A new simple pyrene based schiff base chemosensor 1 (nicotinic acid pyren-1-ylmethylene-hydrazide) has been constructed and is prepared from 1-pyrenecarboxaldehyde and nicotinic hydrazide. Notably, the chemosensor 1 exhibited remarkable colour changes while in the presence of trivalent metal ions like Bi³⁺ & Al³⁺ ion in DMSO-H₂O, (1:1 v/v, HEPES = 50 mM, pH = 7.4). The UV-Vis spectral investigation of chemosensor 1 showed that the maximum absorption peak appeared at 378 nm. In emission studies, chemosensor 1 develops weak fluorescence, while upon the addition of Bi³⁺ and Al³⁺ ions, it exhibits an enhancement of fluorescence intensity. Nevertheless, rest of metal ions have no changes in the emission spectra. The association constant of chemosensor 1 for binding to Bi³⁺ & Al³⁺ system had a value of 1.27 × 10⁴ M^-1 and 1.53 × 10⁴ M^-1. The detection limits were 0.12 µM for Bi³⁺ and 0.17 µM for Al³⁺ respectively. The overall results reveal that chemosensor 1 can act as a dual-channel, highly selective, and sensitive probe for Bi³⁺ and Al³⁺ ions. Moreover, the fluorescence imaging of chemosensor 1 was applied in RAW 264.7 cell line and cytotoxicity assay prove that this chemosensor 1 is non-toxic as well as highly biocompatible.

Keywords: Bi3+ and Al3+ detection; Live cell imaging; PET; Pyrene schiff base; Spectroscopic; Studies.

MeSH terms

Aluminum / analysis*
Animals
Bismuth / analysis*
Fluorescent Dyes / chemistry*
Ions / analysis
Mice
Optical Imaging*
Pyrenes / chemistry*
RAW 264.7 Cells
Spectrometry, Fluorescence

Substances

Fluorescent Dyes
Ions
Pyrenes
pyrene
Aluminum
Bismuth