Identification of the cytochrome P450 involved in the degradation of neonicotinoid insecticide acetamiprid in Phanerochaete chrysosporium

Jianqiao Wang; Haruka Ohno; Yuuri Ide; Hirofumi Ichinose; Toshio Mori; Hirokazu Kawagishi; Hirofumi Hirai

doi:10.1016/j.jhazmat.2019.03.042

Identification of the cytochrome P450 involved in the degradation of neonicotinoid insecticide acetamiprid in Phanerochaete chrysosporium

J Hazard Mater. 2019 Jun 5:371:494-498. doi: 10.1016/j.jhazmat.2019.03.042. Epub 2019 Mar 9.

Authors

Jianqiao Wang¹, Haruka Ohno², Yuuri Ide², Hirofumi Ichinose³, Toshio Mori², Hirokazu Kawagishi⁴, Hirofumi Hirai⁵

Affiliations

¹ Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, School of Environmental Science and Engineering, Guangzhou University, Guangzhou, 510006, China.
² Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan.
³ Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan.
⁴ Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan; Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan; Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan.
⁵ Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan; Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan. Electronic address: hirai.hirofumi@shizuoka.ac.jp.

PMID: 30875576
DOI: 10.1016/j.jhazmat.2019.03.042

Abstract

We previously reported that cytochrome P450 s play critical roles in neonicotinoid insecticide biodegradation by white-rot fungi. Here, we investigated the biodegradation of acetamiprid (ACET) by Phanerochaete chrysosporium to identify the cytochrome P450 involved in this degradation process. During a 20-day incubation period, P. chrysosporium degraded 21% and 51% of ACET in ligninolytic and nonligninolytic media, respectively. The degradation rate of ACET was markedly decreased by the addition of cytochrome P450 inhibitors. Recombinant cytochrome P450s in P. chrysosporium (PcCYP) were heterologously expressed in Saccharomyces cerevisiae strain AH22, and the PcCYP involved in ACET degradation was identified. The results showed that CYP5147A3 can degrade ACET, and two ACET metabolites, N'-cyano-N-methyl acetamidine and 6-chloro-3-pyridinemethanol, were identified. To the best of our knowledge, this study provides the first characterization of the fungal cytochrome P450 that is responsible for the degradation and detoxification of ACET.

Keywords: Acetamiprid; Cytochrome P450; Phanerochaete chrysosporium.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biodegradation, Environmental
Cytochrome P-450 Enzyme System / metabolism*
Insecticides / metabolism*
Neonicotinoids / metabolism*
Phanerochaete / metabolism*

Substances

Insecticides
Neonicotinoids
Cytochrome P-450 Enzyme System