CTCF regulates the FoxO signaling pathway to affect the progression of prostate cancer

Zhengfei Shan; Yongwei Li; Shengqiang Yu; Jitao Wu; Chengjun Zhang; Yue Ma; Guimin Zhuang; Jiantao Wang; Zhenli Gao; Dongfu Liu

doi:10.1111/jcmm.14138

CTCF regulates the FoxO signaling pathway to affect the progression of prostate cancer

J Cell Mol Med. 2019 May;23(5):3130-3139. doi: 10.1111/jcmm.14138. Epub 2019 Mar 15.

Authors

Zhengfei Shan^{1

2}, Yongwei Li², Shengqiang Yu^{1

2}, Jitao Wu², Chengjun Zhang^{1

2}, Yue Ma^{1

2}, Guimin Zhuang^{1

2}, Jiantao Wang^{1

2}, Zhenli Gao^{1

2}, Dongfu Liu^{1

2}

Affiliations

¹ Department of Organ Transplantation, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong, China.
² Department of Urology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong, China.

Abstract

The present research focuses on the influence of CCCTC-binding factor (CTCF) on prostate cancer (PC) via the regulation of the FoxO signalling pathway. A bioinformatics analysis was conducted to screen out target genes for CTCF in LNCaP cells and to enrich the relevant pathways in LNCaP cells. It was found that the FoxO pathway was enriched according to the ChIP-seq results of CTCF. The expression of CTCF, pFoxO1a, FoxO1a, pFoxO3a and FoxO3a was tested by RT-qPCR and Western blot. Inhibition of CTCF could lead to the up-regulation of the FoxO signalling pathway. The rates of cell proliferation, cell invasion and apoptosis were examined by MTT assay, cell invasion assay and flow cytometry under different interference conditions. Down-regulation of CTCF could suppress cell proliferation, cell invasion and facilitate cell apoptosis. Lastly, the effect of CTCF on tumour growth was determined in nude mice. Inhibition of CTCF regulated the FoxO signalling pathway, which retarded tumour growth in vivo. In conclusion, CTCF regulates the FoxO signalling pathway to affect the progress of PC.

Keywords: CTCF; FoxO signaling pathway; prostate cancer.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Apoptosis / genetics
CCCTC-Binding Factor / antagonists & inhibitors
CCCTC-Binding Factor / genetics*
Cell Line, Tumor
Cell Proliferation / genetics
Disease Progression
Forkhead Box Protein O1 / genetics*
Forkhead Box Protein O3 / genetics*
Gene Expression Regulation, Neoplastic / genetics
Heterografts
Humans
Male
Mice
Neoplasm Invasiveness / genetics
Neoplasm Invasiveness / pathology
Prostatic Neoplasms / genetics*
Prostatic Neoplasms / pathology
Signal Transduction / genetics
Transcriptional Activation / genetics

Substances

CCCTC-Binding Factor
CTCF protein, human
FOXO1 protein, human
FOXO3 protein, human
Forkhead Box Protein O1
Forkhead Box Protein O3