In situ fluorescent profiling of living cell membrane proteins at a single-molecule level

Chem Commun (Camb). 2019 Apr 2;55(28):4043-4046. doi: 10.1039/c9cc00244h.

Abstract

We propose a signal amplification method that enables visualization analysis of membrane proteins on living cells at a single-molecule level. Using the proposed method, we achieved imaging of PTK7 membrane proteins on HeLa cells and analyzed the down-regulated expression of EpCAM on the MCF-7 cell surface during epithelial-mesenchymal transition (EMT).

MeSH terms

  • Cell Adhesion Molecules / analysis*
  • Cell Membrane / chemistry*
  • Down-Regulation
  • Epithelial Cell Adhesion Molecule / biosynthesis
  • Epithelial-Mesenchymal Transition
  • Fluorescence*
  • Fluorescent Dyes / chemical synthesis
  • Fluorescent Dyes / chemistry*
  • HeLa Cells
  • Humans
  • MCF-7 Cells
  • Optical Imaging*
  • Receptor Protein-Tyrosine Kinases / analysis*
  • Single Molecule Imaging*

Substances

  • Cell Adhesion Molecules
  • EPCAM protein, human
  • Epithelial Cell Adhesion Molecule
  • Fluorescent Dyes
  • PTK7 protein, human
  • Receptor Protein-Tyrosine Kinases