Crosslinking catalytic hairpin assembly for high-contrast imaging of multiple mRNAs in living cells

Du-Juan Huang; Ting Cao; Zhi-Mei Huang; Zhenkun Wu; Li-Juan Tang; Jian-Hui Jiang

doi:10.1039/c9cc01033e

Crosslinking catalytic hairpin assembly for high-contrast imaging of multiple mRNAs in living cells

Chem Commun (Camb). 2019 Apr 7;55(27):3899-3902. doi: 10.1039/c9cc01033e. Epub 2019 Mar 14.

Authors

Du-Juan Huang¹, Ting Cao¹, Zhi-Mei Huang¹, Zhenkun Wu¹, Li-Juan Tang¹, Jian-Hui Jiang¹

Affiliation

¹ Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China. tomwu@hnu.edu.cn jianhuijiang@hnu.edu.cn.

PMID: 30869692
DOI: 10.1039/c9cc01033e

Abstract

A novel DNA nanotetrad mediated crosslinking catalytic hairpin assembly (CCHA) is reported to generate clumps of cross-linked mesh products for high-contrast and simultaneous imaging of multiple mRNAs in living cells.

MeSH terms

Biosensing Techniques*
Catalysis
Cell Line, Tumor
Cell Survival*
Cross-Linking Reagents / chemical synthesis
Cross-Linking Reagents / chemistry*
DNA / chemistry*
Humans
Nanostructures / chemistry*
Optical Imaging*
RNA, Messenger / analysis*

Substances

Cross-Linking Reagents
RNA, Messenger
DNA