Interstitial Migration of CD8αβ T Cells in the Small Intestine Is Dynamic and Is Dictated by Environmental Cues

Emily A Thompson; Jason S Mitchell; Lalit K Beura; David J Torres; Paulus Mrass; Mark J Pierson; Judy L Cannon; David Masopust; Brian T Fife; Vaiva Vezys

doi:10.1016/j.celrep.2019.02.034

Interstitial Migration of CD8αβ T Cells in the Small Intestine Is Dynamic and Is Dictated by Environmental Cues

Cell Rep. 2019 Mar 12;26(11):2859-2867.e4. doi: 10.1016/j.celrep.2019.02.034.

Authors

Affiliations

¹ Department of Microbiology and Immunology, University of Minnesota, Minneapolis, MN 55455, USA; Center for Immunology, University of Minnesota, Minneapolis, MN 55455, USA.
² Center for Immunology, University of Minnesota, Minneapolis, MN 55455, USA; Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN 55455, USA.
³ Department of Mathematics and Physical Science, Northern New Mexico College, Espanola, NM 87532, USA.
⁴ Department of Molecular Genetics and Microbiology, University of New Mexico, Albuquerque, NM 87131, USA.
⁵ Center for Immunology, University of Minnesota, Minneapolis, MN 55455, USA; Department of Medicine, University of Minnesota, Minneapolis, MN 55455, USA.
⁶ Department of Microbiology and Immunology, University of Minnesota, Minneapolis, MN 55455, USA; Center for Immunology, University of Minnesota, Minneapolis, MN 55455, USA. Electronic address: vvezys@umn.edu.

Abstract

The migratory capacity of adaptive CD8αβ T cells dictates their ability to locate target cells and exert cytotoxicity, which is the basis of immune surveillance for the containment of microbes and disease. The small intestine (SI) is the largest mucosal surface and is a primary site of pathogen entrance. Using two-photon laser scanning microscopy, we found that motility of antigen (Ag)-specific CD8αβ T cells in the SI is dynamic and varies with the environmental milieu. Pathogen-specific CD8αβ T cell movement differed throughout infection, becoming locally confined at memory. Motility was not dependent on CD103 but was influenced by micro-anatomical locations within the SI and by inflammation. CD8 T cells responding to self-protein were initially affected by the presence of self-Ag, but this was altered after complete tolerance induction. These studies identify multiple factors that affect CD8αβ T cell movement in the intestinal mucosa and show the adaptability of CD8αβ T cell motility.

Keywords: 2-photon microscopy; CD8 T cells; T cell migration; immune surveillance; integrins; mathematical modeling; motility; mucosal immunity; resident memory T cells; tolerance.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
CD8-Positive T-Lymphocytes / immunology
CD8-Positive T-Lymphocytes / physiology*
Cell Movement*
Inflammation
Intestine, Small / cytology*
Intestine, Small / immunology
Intestine, Small / pathology
Mice
Mice, Inbred C57BL

Abstract

Publication types

MeSH terms

Grants and funding