A New Red-Emitting Fluorescence Probe for Rapid and Effective Visualization of Bisulfite in Food Samples and Live Animals

Fang Zhou; Yasmina Sultanbawa; Huan Feng; Yong-Lei Wang; Qingtao Meng; Yue Wang; Zhiqiang Zhang; Run Zhang

doi:10.1021/acs.jafc.8b07110

A New Red-Emitting Fluorescence Probe for Rapid and Effective Visualization of Bisulfite in Food Samples and Live Animals

J Agric Food Chem. 2019 Apr 17;67(15):4375-4383. doi: 10.1021/acs.jafc.8b07110. Epub 2019 Mar 25.

Authors

Fang Zhou¹, Yasmina Sultanbawa², Huan Feng¹, Yong-Lei Wang³, Qingtao Meng¹, Yue Wang¹, Zhiqiang Zhang¹, Run Zhang^{1

4}

Affiliations

¹ School of Chemical Engineering , University of Science and Technology Liaoning , Anshan , Liaoning 114051 , People's Republic of China.
² Queensland Alliance for Agricultural and Food Innovation (QAAFI) , The University of Queensland , Brisbane 4072 , Australia.
³ Department of Materials and Environmental Chemistry, Arrhenius Laboratory , Stockholm University , SE-106 91 Stockholm , Sweden.
⁴ Australian Institute for Bioengineering and Nanotechnology , The University of Queensland , Brisbane 4072 , Australia.

PMID: 30865447
DOI: 10.1021/acs.jafc.8b07110

Abstract

The development of new methods for rapid and effective detection of bisulfite (HSO₃^-) in food samples and imaging of HSO₃^- intake in animals is of significant importance due to the key roles of HSO₃^- in food quality assurance and community health. In this work, a new responsive fluorescence probe, EQC, is reported for the quantitative detection of HSO₃^- in food samples and visualization of HSO₃^- intake in animals. Upon addition of HSO₃^-, the UV-vis absorption and red emission of EQC were significantly decreased within 120 s. The changes in absorption and emission spectra of EQC were rationalized by theoretical computations. The proposed reaction mechanism of EQC with HSO₃^- was confirmed by high-resolution mass spectrometry (HRMS) and spectroscopic titration measurements. EQC has the advantages of high sensitivity, selectivity (a detection limit of 18.1 nM), and fast response toward HSO₃^-, which enable rapid and effective HSO₃^- detection in buffer solution. The practical applications of EQC were demonstrated by the detection of HSO₃^- in food samples and the imaging of HSO₃^- intake in live animals.

Keywords: bisulfite; fluorescence probe; food samples; live animals; visualization.

Publication types

Evaluation Study

MeSH terms

Animals
Female
Fluorescence
Fluorescent Dyes / chemistry*
Food Analysis / methods*
Food Contamination / analysis
Limit of Detection
Male
Mice
Mice, Nude
Spectrometry, Fluorescence / methods*
Sulfites / analysis*
Wine / analysis
Zebrafish

Substances

Fluorescent Dyes
Sulfites
hydrogen sulfite