Abstract
The polysaccharides that comprise bacterial cell walls are commonly O-acetylated. This modification confers resistance to hydrolases of innate immune systems and/or controls endogenous autolytic activity. Herein, we present protocols for the compositional analysis of bacterial cell wall O-acetylation, and assays for monitoring O-acetyltransferases and O-acetylesterases. The assays are amenable for the development of high-throughput screens in search of inhibitors of the respective enzymes.
Keywords:
Chitooligosaccharides; O-Acetylation; O-acetylesterase; O-acetyltransferase; Peptidoglycan.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylation
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Acetylesterase / metabolism*
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Acetyltransferases / metabolism*
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Bacteria / chemistry
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Bacteria / enzymology
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Bacteria / metabolism*
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Bacterial Proteins / metabolism*
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Cell Wall / chemistry
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Cell Wall / enzymology
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Cell Wall / metabolism*
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Chromatography, High Pressure Liquid / methods
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Enzyme Assays / methods*
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Mass Spectrometry / methods
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Peptidoglycan / chemistry
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Peptidoglycan / metabolism
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Polysaccharides, Bacterial / chemistry
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Polysaccharides, Bacterial / metabolism*
Substances
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Bacterial Proteins
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Peptidoglycan
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Polysaccharides, Bacterial
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Acetyltransferases
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Acetylesterase