Preventive Effect of Lactobacillus fermentum CQPC08 on 4-Nitroquineline-1-Oxide Induced Tongue Cancer in C57BL/6 Mice

Bihui Liu; Jing Zhang; Ruokun Yi; Xianrong Zhou; Xingyao Long; Yanni Pan; Xin Zhao

doi:10.3390/foods8030093

Preventive Effect of Lactobacillus fermentum CQPC08 on 4-Nitroquineline-1-Oxide Induced Tongue Cancer in C57BL/6 Mice

Foods. 2019 Mar 11;8(3):93. doi: 10.3390/foods8030093.

Authors

Bihui Liu^{1

2

3

4}, Jing Zhang⁵, Ruokun Yi^{6

7

8}, Xianrong Zhou^{9

10}, Xingyao Long^{11

12}, Yanni Pan^{13

14}, Xin Zhao^{15

16

17}

Affiliations

¹ Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, China. liubh@foods.ac.cn.
² Chongqing Engineering Research Center of Functional Food, Chongqing University of Education, Chongqing 400067, China. liubh@foods.ac.cn.
³ Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing University of Education, Chongqing 400067, China. liubh@foods.ac.cn.
⁴ College of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China. liubh@foods.ac.cn.
⁵ Environment and Quality Inspection College, Chongqing Chemical Industry Vocational College, Chongqing 401228, China. zjinger0810@126.com.
⁶ Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, China. yirk@cque.edu.cn.
⁷ Chongqing Engineering Research Center of Functional Food, Chongqing University of Education, Chongqing 400067, China. yirk@cque.edu.cn.
⁸ Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing University of Education, Chongqing 400067, China. yirk@cque.edu.cn.
⁹ Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, China. zhouxr@foods.ac.cn.
¹⁰ College of Food Science, Southwest University, Chongqing 400715, China. zhouxr@foods.ac.cn.
¹¹ Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, China. longyaoyao@foods.ac.cn.
¹² Department of Food Science and Biotechnology, Cha University, Gyeongghi-do, Seongnam 13488, Korea. longyaoyao@foods.ac.cn.
¹³ Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, China. panyanni@foods.ac.cn.
¹⁴ Department of Food Science and Biotechnology, Cha University, Gyeongghi-do, Seongnam 13488, Korea. panyanni@foods.ac.cn.
¹⁵ Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, China. zhaoxin@cque.edu.cn.
¹⁶ Chongqing Engineering Research Center of Functional Food, Chongqing University of Education, Chongqing 400067, China. zhaoxin@cque.edu.cn.
¹⁷ Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing University of Education, Chongqing 400067, China. zhaoxin@cque.edu.cn.

Abstract

Lactobacillus fermentum CQPC08 (LF-CQPC08) is a newly discovered strain of bacteria isolated and identified from traditional pickled vegetables in Sichuan, China. We used 4-nitroquinoline 1-oxide to establish an experimental tongue cancer mouse model to evaluate the preventive effect of LF-CQPC08 on tongue cancer in vivo. Lactobacillus delbruechii subsp. bulgaricus, is a common commercial strain and is used as a positive control to compare the effect with LF-CQPC08. The preventive strength and mechanism of LF-CQPC08 on tongue cancer were determined by measuring the biochemical indicators in mouse serum and tissues. Our results showed LF-CQPC08 inhibits the decline of splenic index, thymus index, percentage of phagocytic macrophages, and phagocytic index effectively. LF-CQPC08 also increased levels of mouse serum granulocyte-colony stimulating factor (G-CSF), granulocyte-macrophage-CSF (GM-CSF), immunoglobulin (Ig)G, IgM levels of serum interleukin (IL)-4, IL-12, tumor necrosis factor-alpha, and interferon-gamma levels, thereby inhibiting the decline in immunity caused by tongue cancer. It also increased the activity levels of superoxide dismutase and glutathione peroxidase and decreased the levels of malondialdehyde in the tissues of the tongue cancer mouse model, thereby suppressing the oxidative stress damage in the tissue caused by tongue cancer. Through quantitative PCR, LF-CQPC08 upregulated the mRNA expression of nuclear factor-erythroid 2 related factor 2 (Nrf2), heme oxygenase-1 (HO-1), glutathione-S-transferases-π (GST-π), and Bcl-2-associated X protein (Bax), and downregulated the mRNA expression of p53, p63, p73, phosphatase and tensin homolog (PTEN), B-cell lymphoma 2 (Bcl-2) and B-cell lymphoma-extra large (Bcl-xL) in the tongue tissues of the tongue cancer mouse. These results indicated that LF-CQPC08 reduced the influence of tongue cancer on the immune system and oxidative balance and improved the immunity and enhanced antioxidant capacity of the mouse model, thereby preventing tongue cancer. LF-CQPC08 could be used as a microbial resource with a preventive effect on tongue cancer.

Keywords: 4-nitroquineline-1-oxide; C57BL/6 mice; Lactobacillus fermentum CQPC08; protein; tongue cancer.