Dissimilatory iron and sulfate reduction by native microbial communities using lactate and citrate as carbon sources and electron donors

Di Xia; Xiaoyun Yi; Yang Lu; Weilin Huang; Yingying Xie; Han Ye; Zhi Dang; Xueqin Tao; Li Li; Guining Lu

doi:10.1016/j.ecoenv.2019.03.005

Dissimilatory iron and sulfate reduction by native microbial communities using lactate and citrate as carbon sources and electron donors

Ecotoxicol Environ Saf. 2019 Jun 15:174:524-531. doi: 10.1016/j.ecoenv.2019.03.005. Epub 2019 Mar 9.

Authors

Di Xia¹, Xiaoyun Yi², Yang Lu³, Weilin Huang⁴, Yingying Xie⁵, Han Ye³, Zhi Dang⁶, Xueqin Tao⁷, Li Li⁷, Guining Lu⁸

Affiliations

¹ School of Environment and Energy, South China University of Technology, Guangzhou 510006, China; South China Institute of Environmental Sciences, Ministry of Environmental Protection, (MEP), Guangzhou 510655, China.
² School of Environment and Energy, South China University of Technology, Guangzhou 510006, China; The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, Guangzhou 510006, China. Electronic address: xyyi@scut.edu.cn.
³ School of Environment and Energy, South China University of Technology, Guangzhou 510006, China.
⁴ School of Environment and Energy, South China University of Technology, Guangzhou 510006, China; School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA.
⁵ School of Environment and Energy, South China University of Technology, Guangzhou 510006, China; School of Chemistry and Environmental Engineering, Hanshan Normal University, Chaozhou 521041, China.
⁶ School of Environment and Energy, South China University of Technology, Guangzhou 510006, China; The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, Guangzhou 510006, China.
⁷ College of Environmental Science and Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
⁸ School of Environment and Energy, South China University of Technology, Guangzhou 510006, China; School of Chemistry and Environmental Engineering, Hanshan Normal University, Chaozhou 521041, China. Electronic address: lutao@scut.edu.cn.

PMID: 30861440
DOI: 10.1016/j.ecoenv.2019.03.005

Abstract

The bacterial (dissimilatory) iron and sulfate reduction (BIR and BSR) are intimately linked to the biogeochemical cycling of C, Fe, and S in acid mine drainage (AMD) environments. This study examined the response of native microbial communities to the reduction of iron and sulfate in bench experimental systems. Results showed that the reduction of ferric iron and sulfate took place when the electron acceptors coexist. Existence of Fe(III) can postpone the reduction of sulfate, but can enhance the reduction rate. Cultures grown in the presence of 10 mM iron can reach the final level of sulfate bio-reduction rate (~100%) after 35 days incubation. 16 S rDNA -based microbial community analysis revealed that the three genera Anaeromusa, Acinetobacter and Bacteroides were dominated in the ferric-reducing conditions. SRB (Desulfobulbus, Desulfosporosinus and Desulfovibrio) were dominated in the sulfate reduction process. Results in this study highlighted the highly coupled nature of C, Fe, and S biogeochemical cycles in AMD and provided insights into the potential of environmental remediation by native microbial.

Keywords: Bacterial iron reduction; Bacterial sulfate reduction; Fermentation; Microbial communities.

MeSH terms

Biodegradation, Environmental
Citric Acid / chemistry*
Electron Transport
Environmental Pollutants / analysis*
Environmental Pollutants / chemistry
Iron / analysis*
Iron / chemistry
Lactic Acid / chemistry*
Microbiota / physiology*
Mining
Models, Theoretical*
RNA, Ribosomal, 16S
Sulfates / analysis*
Sulfates / chemistry
Sulfur-Reducing Bacteria / growth & development

Substances

Environmental Pollutants
RNA, Ribosomal, 16S
Sulfates
Citric Acid
Lactic Acid
Iron