Synthesis and characterization of a novel open cellular Mg-based scaffold for tissue engineering application

Abstract

Tissue engineering is a field which aims to regenerate damaged tissues by enhancing tissue growth through the porous architecture of the scaffolds which is desired to mimic the human cancellous bone. Mg-based scaffolds are gaining importance in the field of tissue engineering owing to its potential application as a biomaterial. However, fabrication of porous Mg remains a daunting task due to its highly reactive nature. In the present work, a novel Mg-based open cell porous structure with pore interconnectivity and significant strength is successfully fabricated using powder metallurgy approach and Ti-woven wire mesh as a space holding material. Pore morphology and percentage porosity can be easily altered by adjusting the Ti-wire diameter and shape of construct. SEM, EDX and µ-CT analysis were performed to assess the microstructural properties of the fabricated scaffold which revealed a uniform distribution of pores with porosity varying in range 50-60%. The measured values of ultimate compressive strength and elastic modulus using quasi static compression test were found to be 101 MPa and 2 GPa, respectively. Further to improve corrosion resistance of fabricated scaffold, alloying and coating were carried out. Preliminary degradation study as well as cytocompatibility studies using L929 cells was carried out to validate the potential of fabricated scaffold for bone healing/repair applications. Fabricated porous structures showed improved corrosion resistance as well as cell viability of more than 90%, suggesting it as a promising development for bone scaffolding applications in future.

Keywords: Biomaterials; Magnesium; Powder metallurgy; Scaffolds; Sintering.