Simultaneous production of methane and acetate by thermophilic mixed culture from carbon dioxide in bioelectrochemical system

Abstract

A thermophilic bioelectrochemical system was operated with mixed culture at 60 °C, while introducing only carbon dioxide. Methane production was initially observed in a membrane-less single chamber without a mediator, but eventually acetate was also found as 10.5 g/L after 137 days of operation. Comparing the microbial communities before and after the electricity supply using next-generation sequencing technology, acetoclastic methanogens such as Methanosaeta concilii were increased, and this result also indicates the production of acetate in bioelectrochemical CO₂ conversion system. With the advent of sulfate-reducing bacteria, Desulfotomaculum peckii was considered to be an acetate production promoter. These high production results for both methane and acetate can be applied to CO₂ storage using excess electricity for value-added chemicals.

Keywords: Bioelectrochemical; Biomethanation; Microbial community.