The effects of dietary supplementation with hyodeoxycholic acid on the differentiation and function of enteroendocrine cells and the serum biochemical indices in weaned piglets1

Enyan Zong; Shanling Yan; Meiwei Wang; Lanmei Yin; Qiye Wang; Jia Yin; Jianzhong Li; Yali Li; Xueqin Ding; Pengfei Huang; Shanping He; Huansheng Yang; Yulong Yin

doi:10.1093/jas/skz059

The effects of dietary supplementation with hyodeoxycholic acid on the differentiation and function of enteroendocrine cells and the serum biochemical indices in weaned piglets1

J Anim Sci. 2019 Feb 11:skz059. doi: 10.1093/jas/skz059. Online ahead of print.

Authors

Enyan Zong¹, Shanling Yan¹, Meiwei Wang¹, Lanmei Yin¹, Qiye Wang¹, Jia Yin¹, Jianzhong Li¹, Yali Li¹, Xueqin Ding¹, Pengfei Huang¹, Shanping He¹, Huansheng Yang^{1

2}, Yulong Yin^{1

2}

Affiliations

¹ Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, Laboratory of Animal Nutrition and Human Health, College of Life Sciences, Hunan Normal University, Changsha, Hunan, China.
² Chinese Academy of Science, Institute of Subtropical Agriculture, Hunan Engineering and Research Center of Animal and Poultry Science and Key Laboratory for Agroecological Processes in Subtropical Region, Scientific Observation and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, Hunan, China.

PMID: 30852589
DOI: 10.1093/jas/skz059

Abstract

Bile acid, a cholesterol metabolite, promotes gastrointestinal tract digestion and absorption of cholesterol, lipids, and fat-soluble vitamins. It is a signaling regulatory molecule that influences a variety of endocrinal and metabolic activities. This study investigated the effects of hyodeoxycholic acid (HDCA) as a dietary supplement on endocrine cell differentiation and function and weaned piglet serum biochemical indices. Sixteen piglets [Duroc × (Landrace × Yorkshire)] were individually housed and weaned at 21 d of age (BW of 6.14 ± 0.22 kg). Uniform weight animals were randomly assigned to 1 of 2 treatments (8 replicate pens per treatment and 1 piglet per pen). The treatments were 1) base diet (control) and 2) base diet supplemented with 2 g/kg of HDCA. Control and HDCA piglet numbers of chromogranin A (CgA)-positive cells per crypt did not differ. HDCA CgA-positive cells numbers decreased (P < 0.05) in the jejunal villi showed a tendency to decrease (P < 0.10) in the ileal villi and showed tendency toward an increase (P < 0.10) in the duodenal villi compared with the controls. The HDCA diet led to a decline in glucagon-like peptide 2 (P < 0.01) concentrations, but did not affect plasma glucagon-like peptide 1. HDCA supplementation increased (P < 0.05) the mRNA expression of jejunal Insm1, Sst, PG, and Gast, but decreased (P < 0.05) duodenal expression of Insm1, jejunal Pdx1, and ileal NeuroD1. HDCA elevated globulin and immunoglobulin A (P < 0.05) serum concentrations and decreased the albumin/globulin ratio (P < 0.05). Total protein and immunoglobulin G serum levels tended to increase compared with the control group. These results indicate that dietary HDCA at 2 g/kg may regulate enteroendocrine cell differentiation and play a role in increasing weaned piglet humoral immunity.

Keywords: cell differentiation; enteroendocrine cell; hyodeoxycholic acid; intestine; weaned piglet.