Facile strategy to prepare a metalloporphyrin-based hydrophilic porous organic polymer with enhanced peroxidase-like activity and high stability for colorimetric detection of H2O2 and glucose

Tingting Liu; Jing Tian; Lin Cui; Qingyun Liu; Lili Wu; Xiaomei Zhang

doi:10.1016/j.colsurfb.2019.03.008

Facile strategy to prepare a metalloporphyrin-based hydrophilic porous organic polymer with enhanced peroxidase-like activity and high stability for colorimetric detection of H₂O₂ and glucose

Colloids Surf B Biointerfaces. 2019 Jun 1:178:137-145. doi: 10.1016/j.colsurfb.2019.03.008. Epub 2019 Mar 4.

Authors

Tingting Liu¹, Jing Tian², Lin Cui³, Qingyun Liu⁴, Lili Wu⁵, Xiaomei Zhang⁶

Affiliations

¹ School of Chemistry and Chemical Engineering, Shandong University, Jinan, Shandong 250100, China.
² Shandong Product Quality Inspection Research Institute, Jinan, Shandong 250100, China.
³ College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan, Shandong 250014, China.
⁴ School of Chemistry and Environmental Engineering, Shandong University of Science and Technology, Qingdao, Shandong 266590, China.
⁵ School of materials science and engineering, Shandong University, Jinan, Shandong 250100, China.
⁶ School of Chemistry and Chemical Engineering, Shandong University, Jinan, Shandong 250100, China. Electronic address: zhangxiaomei@sdu.edu.cn.

PMID: 30852265
DOI: 10.1016/j.colsurfb.2019.03.008

Abstract

Nanozymes, nanomaterial-based artificial enzymes, have attracted researchers' enormous interest due to their unique properties compared with natural enzymes. To mimic the catalytic function of natural enzymes, designing high-efficient, novel nanozymes is crucial yet challenging task. In this article, we described the synthesis and functions of a metalloporphyrin-based porous organic polymer, namely FePPOPs-SO₃H. FePPOPs-SO₃H was synthesized effortlessly via an extensive aromatic electrophilic substitution and the following sulfonation reactions. This strategy was cost-efficient without the participation of precious metal catalysts. The resultant FePPOPs-SO₃H is intriguing since the framework itself is constructed by covalently linked porphyrin units, which could serve as a built-in catalyst and strengthen the stability of polymer. With sulfonic acid side groups, FePPOPs-SO₃H is well water-dispersive. Owing to these unique characteristics, FePPOPs-SO₃H exhibited excellent peroxidase-like activity toward a classical peroxidase substrate 3,3',5,5'-tetramethylbenzidine (TMB) to produce a blue product only within 20 s. The peroxidase-mimicking performance of FePPOPs-SO₃H outperforms the ferric porphyrin monomer and normal Fe₃O₄ nanoparticles. Based on the excellent catalytic activity of FePPOPs-SO₃H, two visual colorimetric sensors for ultrafast detecting H₂O₂ and glucose, respectively, were constructed with a wide linear range of 50-1800 μM (for H₂O₂) and 200-1500 μM (for glucose), as well as a relative lower limit of detection (LOD) [26.70 μM (for H₂O₂) and 16.38 μM (for glucose)]. Our strategy highlights opportunities for the design of new metalloporphyrin-based porous organic polymers with built-in catalytic skeletons and inherently excellent peroxidase-mimicking performance.

Keywords: Colorimetric; Metalloporphyrin; Nanozymes; Peroxidase-like; Porous organic polymers.

MeSH terms

Colorimetry
Glucose / chemistry*
Hydrogen Peroxide / chemistry*
Hydrophobic and Hydrophilic Interactions
Metalloporphyrins / chemistry*
Peroxidase / metabolism
Polymers / chemistry*

Substances

Metalloporphyrins
Polymers
Hydrogen Peroxide
Peroxidase
Glucose