A biosurfactant-producing Pseudomonas aeruginosa S5 isolated from coking wastewater and its application for bioremediation of polycyclic aromatic hydrocarbons

Shengli Sun; Yuxiao Wang; Tingting Zang; Jingyue Wei; Haizhen Wu; Chaohai Wei; Guanglei Qiu; Fusheng Li

doi:10.1016/j.biortech.2019.02.087

A biosurfactant-producing Pseudomonas aeruginosa S5 isolated from coking wastewater and its application for bioremediation of polycyclic aromatic hydrocarbons

Bioresour Technol. 2019 Jun:281:421-428. doi: 10.1016/j.biortech.2019.02.087. Epub 2019 Feb 20.

Authors

Shengli Sun¹, Yuxiao Wang¹, Tingting Zang², Jingyue Wei³, Haizhen Wu⁴, Chaohai Wei⁵, Guanglei Qiu⁶, Fusheng Li³

Affiliations

¹ School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, PR China.
² State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 51006, PR China.
³ School of Environment and Energy, South China University of Technology, Guangzhou 510006, PR China; River Basin Research Center, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
⁴ School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, PR China. Electronic address: hzhwu2@scut.edu.cn.
⁵ School of Environment and Energy, South China University of Technology, Guangzhou 510006, PR China; State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 51006, PR China.
⁶ School of Environment and Energy, South China University of Technology, Guangzhou 510006, PR China.

PMID: 30849698
DOI: 10.1016/j.biortech.2019.02.087

Abstract

Although polycyclic aromatic hydrocarbons (PAHs) are considered as toxic and refractory pollutants, their biodegradation can be facilitated by biosurfactants. However, few studies have been performed to understand the potential isolation and application of biosurfactant-producing microorganism for promoting the in-situ removal of PAHs from wastewaters. In this work, a biosurfactant-producing strain S5 isolated from coking wastewater was identified as Pseudomonas aeruginosa. The biosurfactant produced by strain S5 was determined as glycolipid with a critical micelle concentration (CMC) of 96.5 mg/L, and reduced the surface tension from 72.2 to 29.6 mN/m. Addition indigenous P. aeruginosa S5 to coking wastewater effectively promoted the biodegradation of high weight molecular (HWM) PAHs (reduction from 9141.02 to 5117.16 µg/L in 15 days) in sludge phase. The results showed that the removal of PAHs in the sludge was enhanced by inoculating indigenous biosurfactant-producing microorganism in coking wastewater serving as an in-site remediation technology.

Keywords: Biosurfactant-producing microorganism; Coking wastewater; PAHs bioremediation; Pseudomonas aeruginosa.

MeSH terms

Biodegradation, Environmental
Coke*
Environmental Restoration and Remediation
Polycyclic Aromatic Hydrocarbons / metabolism*
Pseudomonas aeruginosa / isolation & purification
Pseudomonas aeruginosa / metabolism*
Surface-Active Agents / metabolism
Wastewater / microbiology*

Substances

Coke
Polycyclic Aromatic Hydrocarbons
Surface-Active Agents
Waste Water