Gad1-promotor-driven GFP expression in non-GABAergic neurons of the nucleus endopiriformis in a transgenic mouse line

Sophie Riedemann; Bernd Sutor; Matteo Bergami; Therese Riedemann

doi:10.1002/cne.24673

Gad1-promotor-driven GFP expression in non-GABAergic neurons of the nucleus endopiriformis in a transgenic mouse line

J Comp Neurol. 2019 Oct 1;527(14):2215-2232. doi: 10.1002/cne.24673. Epub 2019 Mar 20.

Authors

Sophie Riedemann¹, Bernd Sutor¹, Matteo Bergami^{2

3}, Therese Riedemann¹

Affiliations

¹ Department of Physiological Genomics, Biomedical Center, Ludwig-Maximilians-University, Munich, Germany.
² Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD) and University Hospital of Cologne, Cologne, Germany.
³ Center for Molecular Medicine (CMMC), University of Cologne, Cologne, Germany.

PMID: 30847931
DOI: 10.1002/cne.24673

Abstract

Transgenic animals have become a widely used model to identify and study specific cell types in whole organs. Promotor-driven reporter gene labeling of the cells under investigation has promoted experimental efficacy to a large degree. However, rigorous assessment of transgene expression specificity in these animal models is highly recommended to validate cellular identity and to isolate potentially mislabeled cell populations. Here, we report on one such mislabeled neuron population in a widely used transgenic mouse line in which GABAergic somatostatin-expressing interneurons (SOM^pos INs) are labeled by eGFP (so-called GIN mouse, FVB-Tg(GadGFP)45704Swn/J). These neurons represent a subpopulation of all SOM^pos INs. However, we report here on GFP labeling of non-GABAergic neurons in the nucleus endopiriformis of this mouse line.

Keywords: GABA; GAD67-promotor; RRID:AB_10000343; RRID:AB_10562708; RRID:AB_10563568; RRID:AB_10563770; RRID:AB_11180061; RRID:AB_2057032; RRID:AB_2255365; RRID:AB_2307443; RRID:AB_2338013; RRID:AB_2338078; RRID:AB_2338251; RRID:AB_2338680; RRID:AB_2338928; RRID:AB_258984; RRID:AB_310288; RRID:AB_477019; eGFP; interneurons; nucleus endopiriformis; off-target labeling.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Claustrum / chemistry
Claustrum / metabolism*
GABAergic Neurons / chemistry
GABAergic Neurons / metabolism*
Gene Expression
Glutamate Decarboxylase / analysis
Glutamate Decarboxylase / biosynthesis*
Glutamate Decarboxylase / genetics
Green Fluorescent Proteins / analysis
Green Fluorescent Proteins / biosynthesis*
Green Fluorescent Proteins / genetics
Mice
Mice, Transgenic
Organ Culture Techniques
Piriform Cortex / chemistry
Piriform Cortex / metabolism*

Substances

Green Fluorescent Proteins
Glutamate Decarboxylase
glutamate decarboxylase 1