Multi-Epitope Fusion Protein Eg mefAg-1 as a Serodiagnostic Candidate for Cystic Echinococcosis in Sheep

Korean J Parasitol. 2019 Feb;57(1):61-67. doi: 10.3347/kjp.2019.57.1.61. Epub 2019 Feb 26.

Abstract

Cystic echinococcosis (CE) in sheep is a hazardous zoonotic parasitic disease that is caused by Echinococcus granulosus (Eg). At present, serological test is an important diagnostic method for Eg infection in domestic animals. Here, a fusion protein Eg mefAg-1 harboring 8 dominant B-cell epitopes of Eg such as antigen B, tetraspanin 1, tetraspanin 6, reticulon and Eg95 was produced in E. coli and evaluated for CE in sheep by indirect ELISA. Eg mefAg-1 showed in ELISA a high sensitivity (93.41%) and specificity (99.31%), with a coincidence rate of 97.02%. Overall, it is suggested that the Eg mefAg-1 could be a potential antigen candidate for CE serodiagnosis in sheep.

Keywords: antigen; cystic echinococcosis; fusion protein; indirect ELISA; multi-epitope; serodiagnostic; sheep.

Publication types

  • Evaluation Study

MeSH terms

  • Animals
  • Antibodies, Helminth / blood*
  • Antigens, Helminth / genetics
  • Antigens, Helminth / immunology*
  • Echinococcosis / diagnosis
  • Echinococcosis / parasitology
  • Echinococcosis / veterinary*
  • Echinococcus / immunology
  • Echinococcus / isolation & purification*
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Epitopes / genetics
  • Epitopes / immunology
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Incidence
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / immunology
  • Sensitivity and Specificity
  • Serologic Tests / methods*
  • Sheep
  • Sheep Diseases / diagnosis*
  • Sheep Diseases / parasitology

Substances

  • Antibodies, Helminth
  • Antigens, Helminth
  • Epitopes
  • Recombinant Fusion Proteins